Hotspot

psbM-trnD-GUC

LSC · 31363-32360 · 998 bp

Back to Sassafras

Pi 0.00673

MarkerSeek score 42.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 998
variable_sites: 10
pi: 0.00673
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	42.9	50.0	58.2	57.9	1108-1111 bp	1.0	70.7
psbM-trnD-GUC_p2	GAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	56.7	57.9	1107-1110 bp	1.0	70.5
psbM-trnD-GUC_p3	GAATGAACAACGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	42.9	50.0	57.5	57.9	1107-1110 bp	1.0	68.2
psbM-trnD-GUC_p4	AGAATGAACAACGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	56.5	57.9	1108-1111 bp	1.0	68.2
psbM-trnD-GUC_p5	AATGAACAACGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	40.0	50.0	56.3	57.9	1106-1109 bp	1.0	66.7