Hotspot

rpoB-trnC-GCA

LSC · 27829-28976 · 1148 bp

Back to Sassafras

Pi 0.00407

MarkerSeek score 61.40

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 7
pi: 0.00407
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGTTCTTCCATCAAGCCTTGA	TTGTTAATCAGGCGACACCC	42.9	50.0	57.2	58.2	1307-1308 bp	1.0	64.7
rpoB-trnC-GCA_p2	TGAAGTTCTTCCATCAAGCCT	TTGTTAATCAGGCGACACCC	42.9	50.0	57.2	58.2	1310-1311 bp	1.0	64.7
rpoB-trnC-GCA_p3	AGTTCTTCCATCAAGCCTTGA	CTGGGGAGAAAGGATTTGCA	42.9	50.0	57.2	57.8	1278-1279 bp	1.0	64.4
rpoB-trnC-GCA_p4	AGTTCTTCCATCAAGCCTTGA	TGGGGAGAAAGGATTTGCAG	42.9	50.0	57.2	57.8	1277-1278 bp	1.0	64.4
rpoB-trnC-GCA_p5	TGAAGTTCTTCCATCAAGCCT	TGGGGAGAAAGGATTTGCAG	42.9	50.0	57.2	57.8	1280-1281 bp	1.0	64.4