primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	96	490	457-461	1.000000	81.2
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.199180	96	490	471-475	1.000000	81.1
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	96	490	470-474	1.000000	81.1
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	20	20	50.0	55.0	57.9	57.9	0.0	13.4	0.200688	96	490	472-476	1.000000	81.1
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.203648	96	490	460-464	1.000000	81.1
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4372	5188	947-949	1.000000	79.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.2	58.0	20.3	7.6	0.192760	4372	5188	946-948	1.000000	79.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAAGACCATAGCCGCACTT	AAAAAGGCGCTCAACCTACA	20	20	45.0	45.0	57.8	58.0	0.0	7.6	0.224713	4372	5188	968-970	1.000000	78.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CCGCACTTAAAAGCCGAGTA	AAAAAGGCGCTCAACCTACA	20	20	50.0	45.0	58.3	58.0	5.3	7.6	0.307146	4372	5188	956-958	1.000000	78.1
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	CTTGAAAAAGGCGCTCAACC	20	20	50.0	50.0	58.2	58.2	20.3	7.6	0.397463	4372	5188	950-952	1.000000	77.4
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	AGTTCTTCCATCAAGCCTTGA	TTGTTAATCAGGCGACACCC	21	20	42.9	50.0	57.2	58.2	10.0	0.0	1.979613	27829	28976	1307-1308	1.000000	64.7
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TGAAGTTCTTCCATCAAGCCT	TTGTTAATCAGGCGACACCC	21	20	42.9	50.0	57.2	58.2	4.2	0.0	1.979613	27829	28976	1310-1311	1.000000	64.7
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	AGTTCTTCCATCAAGCCTTGA	CTGGGGAGAAAGGATTTGCA	21	20	42.9	50.0	57.2	57.8	10.0	0.0	2.016340	27829	28976	1278-1279	1.000000	64.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	AGTTCTTCCATCAAGCCTTGA	TGGGGAGAAAGGATTTGCAG	21	20	42.9	50.0	57.2	57.8	10.0	0.0	2.016340	27829	28976	1277-1278	1.000000	64.4
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TGAAGTTCTTCCATCAAGCCT	TGGGGAGAAAGGATTTGCAG	21	20	42.9	50.0	57.2	57.8	4.2	0.0	2.016340	27829	28976	1280-1281	1.000000	64.4
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	21	20	42.9	50.0	58.2	57.9	0.0	0.0	1.319742	31363	32360	1108-1111	1.000000	70.7
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	GAATGAACAACGCAGTAGCA	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	56.7	57.9	0.0	0.0	1.336812	31363	32360	1107-1110	1.000000	70.5
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	GAATGAACAACGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	21	20	42.9	50.0	57.5	57.9	0.0	0.0	1.622212	31363	32360	1107-1110	1.000000	68.2
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGAATGAACAACGCAGTAGC	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	56.5	57.9	0.0	0.0	1.630110	31363	32360	1108-1111	1.000000	68.2
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AATGAACAACGCAGTAGCAA	AGTTCAATCGGTCAGAGCAC	20	20	40.0	50.0	56.3	57.9	0.0	0.0	1.813464	31363	32360	1106-1109	1.000000	66.7
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CATGATCAACCCCCTTTCGT	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.134741	32959	33679	870-872	1.000000	79.5
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	CAACCCCCTTTCGTCTTACC	GAACCGATGACTTACGCCTT	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.188613	32959	33679	864-866	1.000000	79.0
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CATGATCAACCCCCTTTCGT	CCATGGCGTTACTCTACCAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.192838	32959	33679	849-851	1.000000	79.0
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	CATGATCAACCCCCTTTCGT	TGGCGTTACTCTACCACTGA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.214993	32959	33679	846-848	1.000000	78.8
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CAACCCCCTTTCGTCTTACC	CCATGGCGTTACTCTACCAC	20	20	55.0	55.0	58.2	58.1	0.0	0.0	0.246710	32959	33679	843-845	1.000000	78.6
psbC_p1	psbC	1	TTCGTTTCCCAGGAAATCCG	TCATGCATTTGATCTCCTGTC	20	21	50.0	42.9	58.2	56.0	14.7	0.0	3.156661	36208	37629	1558-1558	1.000000	54.9
psbC_p2	psbC	2	GCAGCGGAAGATCCTGAATT	TCATGCATTTGATCTCCTGTC	20	21	50.0	42.9	58.3	56.0	0.6	0.0	3.299567	36208	37629	1537-1537	1.000000	53.8
psbC_p3	psbC	3	AGGTATTCGTGCTTGGATGG	TCATGCATTTGATCTCCTGTC	20	21	50.0	42.9	57.7	56.0	0.0	0.0	3.304798	36208	37629	1481-1481	1.000000	53.7
psbC_p4	psbC	4	TTCGTTTCCCAGGAAATCCG	CTTCATGCATTTGATCTCCTGTC	20	23	50.0	43.5	58.2	57.8	14.7	0.0	3.355073	36208	37629	1560-1560	1.000000	53.3
psbC_p5	psbC	5	TTCGTTTCCCAGGAAATCCG	TTCATGCATTTGATCTCCTGTC	20	22	50.0	40.9	58.2	56.8	14.7	0.0	3.424770	36208	37629	1559-1559	1.000000	52.8
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TCTCCTGAGGTTGTCGGAAT	TTGTGGTCGGTTGGTGAATT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.121466	46706	47514	1022-1022	1.000000	79.3
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TCTCCTGAGGTTGTCGGAAT	ACGGAAAGAGAGGGATTCGA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.212764	46706	47514	992-992	1.000000	78.6
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TCTCCTGAGGTTGTCGGAAT	TTCCAATGCTACGCCTTGAA	20	20	50.0	45.0	58.1	57.8	0.0	5.6	0.254079	46706	47514	942-942	1.000000	78.3
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TCTCCTGAGGTTGTCGGAAT	ATTGTGGTCGGTTGGTGAAT	20	20	50.0	45.0	58.1	57.7	0.0	0.0	0.347780	46706	47514	1023-1023	1.000000	77.5
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TTCTCCTGAGGTTGTCGGAA	TTGTGGTCGGTTGGTGAATT	20	20	50.0	45.0	58.3	57.9	0.0	0.0	0.347994	46706	47514	1023-1023	1.000000	77.5
petA-psbJ_p1	petA-psbJ	1	ACGTGTCCAAGGTTTGTTGT	GGCTCATATTCCGGATTGGG	20	20	45.0	55.0	58.2	58.2	0.0	4.1	0.344636	66363	67498	1257-1281	1.000000	83.1
petA-psbJ_p2	petA-psbJ	2	ACGTGTCCAAGGTTTGTTGT	CGGCTCATATTCCGGATTGG	20	20	45.0	55.0	58.2	58.6	0.0	4.1	0.728252	66363	67498	1258-1282	1.000000	80.0
petA-psbJ_p3	petA-psbJ	3	ACGTGTCCAAGGTTTGTTGT	GCTCATATTCCGGATTGGGA	20	20	45.0	50.0	58.2	57.1	0.0	4.1	1.030186	66363	67498	1256-1280	1.000000	77.6
petA-psbJ_p4	petA-psbJ	4	ACGTGTCCAAGGTTTGTTGT	ATTCCGGATTGGGATCATCC	20	20	45.0	50.0	58.2	57.1	0.0	4.1	1.058172	66363	67498	1250-1274	1.000000	77.4
petA-psbJ_p5	petA-psbJ	5	CGTGTCCAAGGTTTGTTGTT	GGCTCATATTCCGGATTGGG	20	20	45.0	55.0	57.1	58.2	0.0	4.1	1.105127	66363	67498	1256-1280	1.000000	77.0
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.527577	68268	69511	1391-1424	1.000000	75.9
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GCAGCCAGTAGAAAACCGAA	20	20	50.0	50.0	57.9	58.5	6.4	0.0	0.531926	68268	69511	1389-1422	1.000000	75.9
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AAGCAGCCAGTAGAAAACCG	20	20	45.0	50.0	58.2	58.5	0.0	0.0	0.647263	68268	69511	1400-1433	1.000000	75.0
psbE-petL_p4	psbE-petL	4	ACGAATAACCAACCCGCAAT	GCAGCCAGTAGAAAACCGAA	20	20	45.0	50.0	58.2	58.5	0.0	0.0	0.651612	68268	69511	1398-1431	1.000000	75.0
psbE-petL_p5	psbE-petL	5	TGCTGACGAATAACCAACCC	AAGCAGCCAGTAGAAAACCG	20	20	50.0	50.0	58.2	58.5	0.0	0.0	0.660168	68268	69511	1405-1438	1.000000	74.9
clpP-psbB_p1	clpP-psbB	1	CTTTTGGAACACCAATGGGC	ATATGGACAGAAAGCAGCCG	20	20	50.0	50.0	57.8	58.0	3.6	0.0	0.209284	75561	75988	521-521	1.000000	78.9
clpP-psbB_p2	clpP-psbB	2	ACCCAAACTGCATCTTCCTC	ATATGGACAGAAAGCAGCCG	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.247535	75561	75988	561-561	1.000000	78.6
clpP-psbB_p3	clpP-psbB	3	ATACGTCAACCCAAACTGCA	ATATGGACAGAAAGCAGCCG	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.304963	75561	75988	569-569	1.000000	78.1
clpP-psbB_p4	clpP-psbB	4	TACGTCAACCCAAACTGCAT	ATATGGACAGAAAGCAGCCG	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.304963	75561	75988	568-568	1.000000	78.1
clpP-psbB_p5	clpP-psbB	5	TTTGGAACACCAATGGGCAT	ATATGGACAGAAAGCAGCCG	20	20	45.0	50.0	58.3	58.0	3.6	0.0	0.313896	75561	75988	519-519	1.000000	78.1
rpoA_p1	rpoA	1	AGGATTCAAAAGCCATTTTCTTCT	CATAATGGATGTAGGCCTCCT	24	21	33.3	47.6	57.3	56.6	0.0	9.7	7.044706	81458	82477	1190-1195	1.000000	40.3
rpoA_p2	rpoA	2	AGGATTCAAAAGCCATTTTCTTCT	TGGATGTAGGCCTCCTAAAA	24	20	33.3	45.0	57.3	55.5	0.0	9.7	7.170945	81458	82477	1185-1190	1.000000	40.3
rpoA_p3	rpoA	3	AGGATTCAAAAGCCATTTTCTTCT	TGGATGTAGGCCTCCTAAAAA	24	21	33.3	42.9	57.3	56.3	0.0	9.7	7.355602	81458	82477	1185-1190	1.000000	40.3
rpoA_p4	rpoA	4	AGGATTCAAAAGCCATTTTCTTC	CATAATGGATGTAGGCCTCCT	23	21	34.8	47.6	56.0	56.6	0.0	9.7	7.405644	81458	82477	1190-1195	1.000000	40.3
rpoA_p5	rpoA	5	GGATTCAAAAGCCATTTTCTTCT	CATAATGGATGTAGGCCTCCT	23	21	34.8	47.6	56.0	56.6	0.0	9.7	7.405644	81458	82477	1189-1194	1.000000	40.3
ycf2_p1	ycf2	1	TGAGTCACAGGTATCTGGCA	TCCGTTGTGGAAGAATCGAC	20	20	50.0	50.0	58.1	57.9	8.1	0.0	0.137356	93448	93567	638-638	1.000000	80.3
ycf2_p2	ycf2	2	TGAGTCACAGGTATCTGGCA	TGATTTGACCCCATAAGCGG	20	20	50.0	50.0	58.1	57.9	8.1	0.0	0.114143	93448	93567	1081-1081	1.000000	80.0
ycf2_p3	ycf2	3	TGAGTCACAGGTATCTGGCA	CCACCACCCAATAGCATGTT	20	20	50.0	50.0	58.1	58.1	8.1	0.0	0.211505	93448	93567	1059-1059	1.000000	79.2
ycf2_p4	ycf2	4	AGACATTTCTGGAACGCCTG	TGATTTGACCCCATAAGCGG	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.237821	93448	93567	952-952	0.333333	51.4
ycf2_p5	ycf2	5	CAGACATTTCTGGAACGCCT	TGATTTGACCCCATAAGCGG	20	20	50.0	50.0	58.2	57.9	0.6	0.0	0.237821	93448	93567	953-953	0.333333	51.4
ndhF-rpl32_p1	ndhF-rpl32	1	GCGAAAGGTATGATCCATGC	GTACGTTTCTTTGGAACCGC	20	20	50.0	50.0	57.0	57.7	0.0	0.0	1.282656	115898	117121	1288-1307	1.000000	71.0
ndhF-rpl32_p2	ndhF-rpl32	2	GCGAAAGGTATGATCCATGC	GGCCTGATATCCCCTTCCTT	20	20	50.0	55.0	57.0	58.5	0.0	0.0	1.527045	115898	117121	1346-1365	1.000000	69.0
ndhF-rpl32_p3	ndhF-rpl32	3	GCGAAAGGTATGATCCATGC	TACGTTTCTTTGGAACCGCC	20	20	50.0	50.0	57.0	58.8	0.0	0.0	1.748872	115898	117121	1287-1306	1.000000	67.3
ndhF-rpl32_p4	ndhF-rpl32	4	AGCGAAAGGTATGATCCATGC	GTACGTTTCTTTGGAACCGC	21	20	47.6	50.0	58.5	57.7	0.0	0.0	1.807085	115898	117121	1289-1308	1.000000	66.8
ndhF-rpl32_p5	ndhF-rpl32	5	AGCGAAAGGTATGATCCATGC	GGCCTGATATCCCCTTCCTT	21	20	47.6	55.0	58.5	58.5	0.0	0.0	2.051473	115898	117121	1347-1366	1.000000	64.8
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	CGCGGCAAAAGCTTTATCTT	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.7	57.9	13.1	0.0	0.386849	117296	118669	1507-1517	1.000000	77.8
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	CGCGGCAAAAGCTTTATCTT	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	57.7	57.8	13.1	0.0	0.447576	117296	118669	1500-1510	1.000000	77.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	CGCGGCAAAAGCTTTATCTT	CCTAAGAGCAGCGTGTCTAC	20	20	45.0	55.0	57.7	57.8	13.1	0.0	0.458867	117296	118669	1506-1516	1.000000	77.2
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	CGCGGCAAAAGCTTTATCTT	CTAAGAGCAGCGTGTCTACC	20	20	45.0	55.0	57.7	57.8	13.1	0.0	0.458867	117296	118669	1505-1515	1.000000	77.2
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	CGCGGCAAAAGCTTTATCTT	GAGCAGCGTGTCTACCAATT	20	20	45.0	50.0	57.7	58.3	13.1	0.0	0.535719	117296	118669	1501-1511	1.000000	76.6
ndhA_p1	ndhA	1	CCTTGCAGCTCGTATTGTCT	TTTGGAACGAGTTGAAGGGG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.040076	124185	126399	2940-2942	1.000000	80.2
ndhA_p2	ndhA	2	CCTTGCAGCTCGTATTGTCT	GGTTTATCGGGACCAATGCT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.068518	124185	126399	2885-2887	1.000000	80.0
ndhA_p3	ndhA	3	AACCCAGTCACCATAGGGAA	TTTGGAACGAGTTGAAGGGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.078087	124185	126399	2903-2905	1.000000	79.9
ndhA_p4	ndhA	4	AACCCAGTCACCATAGGGAA	GGTTTATCGGGACCAATGCT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.106529	124185	126399	2848-2850	1.000000	79.7
ndhA_p5	ndhA	5	CCTTGCAGCTCGTATTGTCT	GGAAAATTCGTCCACCTGGT	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.110098	124185	126399	2482-2484	1.000000	79.6
rps15-ycf1_p1	rps15-ycf1	1	TGAAATTCAACAGACCCTTTGT	AATGGCAGCCGTTTCAGTAT	22	20	36.4	45.0	56.4	57.9	0.0	13.7	3.755555	127957	128276	448-450	1.000000	51.6
rps15-ycf1_p2	rps15-ycf1	2	TGAAATTCAACAGACCCTTTGT	ATAATGGCAGCCGTTTCAGT	22	20	36.4	45.0	56.4	57.9	0.0	24.3	3.755555	127957	128276	450-452	1.000000	51.6
rps15-ycf1_p3	rps15-ycf1	3	ACTTGAAATTCAACAGACCCT	AATGGCAGCCGTTTCAGTAT	21	20	38.1	45.0	55.3	57.9	4.0	13.7	3.813526	127957	128276	451-453	1.000000	51.1
rps15-ycf1_p4	rps15-ycf1	4	ACTTGAAATTCAACAGACCCT	ATAATGGCAGCCGTTTCAGT	21	20	38.1	45.0	55.3	57.9	4.0	24.3	3.813526	127957	128276	453-455	1.000000	51.1
rps15-ycf1_p5	rps15-ycf1	5	TGAAATTCAACAGACCCTTTGT	CCAATAATGGCAGCCGTTTC	22	20	36.4	50.0	56.4	57.8	0.0	24.3	3.835110	127957	128276	453-455	1.000000	50.9
ycf1_p1	ycf1	1	AGTCGAAATAAAGTGCATTTT	TTCATACGCAAATGGCACTA	21	20	28.6	40.0	52.3	55.5	12.2	0.0	9.218089	130347	130466	195-195	1.000000	42.5
ycf1_p2	ycf1	2	AGTCGAAATAAAGTGCATTTT	TCATACGCAAATGGCACTAT	21	20	28.6	40.0	52.3	55.2	12.2	0.0	9.452213	130347	130466	194-194	1.000000	42.5
ycf1_p3	ycf1	3	AGTCGAAATAAAGTGCATTTT	TCATACGCAAATGGCACTATT	21	21	28.6	38.1	52.3	56.0	12.2	0.0	9.646911	130347	130466	194-194	1.000000	42.5
ycf1_p4	ycf1	4	AGTCGAAATAAAGTGCATTTT	ATTCATACGCAAATGGCACT	21	20	28.6	40.0	52.3	56.1	12.2	0.0	8.581273	130347	130466	196-196	1.000000	42.4
ycf1_p5	ycf1	5	TAGTCGAAATAAAGTGCATTTT	ATTCATACGCAAATGGCACT	22	20	27.3	40.0	52.4	56.1	12.2	0.0	9.481857	130347	130466	197-197	1.000000	42.4