Hotspot

atpF

LSC · 34602-36012 · 1411 bp

Back to Sasamorpha

Pi 0.00335

MarkerSeek score 35.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1411
variable_sites: 7
pi: 0.00335
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 35.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	AGAGGATATGCTCGCCTTCT	CATCAACCCATCGCCCATTA	50.0	50.0	58.0	58.0	2001-2019 bp	1.0	80.9
atpF_p2	GTATTGCGAGACAGCCAGAA	CATCAACCCATCGCCCATTA	50.0	50.0	58.0	58.0	2319-2337 bp	1.0	80.7
atpF_p3	AGAGGATATGCTCGCCTTCT	TTTTGCCAGTCTGTCTGTCC	50.0	50.0	58.0	58.0	2284-2302 bp	1.0	80.7
atpF_p4	AGAGGATATGCTCGCCTTCT	CAATTTCGCCTCTCCCATCA	50.0	50.0	58.0	58.0	2122-2140 bp	1.0	80.6
atpF_p5	GTATTGCGAGACAGCCAGAA	TTTTGCCAGTCTGTCTGTCC	50.0	50.0	58.0	58.0	2602-2620 bp	1.0	80.5