Hotspot

petD-rpoA

LSC · 76037-76277 · 241 bp

Back to Sasamorpha

Pi 0.0313

MarkerSeek score 61.60

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 241
variable_sites: 10
pi: 0.0313
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	ATCGGTACTGCAGTAGCTCT	TGGGACACTCTAGAGAAGCA	50.0	50.0	57.9	57.4	354-382 bp	1.0	80.9
petD-rpoA_p2	TCGGTACTGCAGTAGCTCTT	TGGGACACTCTAGAGAAGCA	50.0	50.0	58.2	57.4	353-381 bp	1.0	80.1
petD-rpoA_p3	ATCGGTACTGCAGTAGCTCT	GAGAAGCATCTCCCAATCGA	50.0	50.0	57.9	57.1	342-370 bp	1.0	78.6
petD-rpoA_p4	CCAGTAGCTACGACCGTTTT	TGGGACACTCTAGAGAAGCA	50.0	50.0	57.6	57.4	378-406 bp	1.0	78.4
petD-rpoA_p5	TCGGTACTGCAGTAGCTCTT	GAGAAGCATCTCCCAATCGA	50.0	50.0	58.2	57.1	341-369 bp	1.0	77.8