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clpP-psbB

LSC · 70224-70717 · 494 bp

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Pi 0.0027

MarkerSeek score 52.90

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 2
pi: 0.0027
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCCAAGTCGCTTCTTCATC	TATGCACCGAAAGCAATCGA	50.0	45.0	57.9	58.0	625-626 bp	1.0	79.4
clpP-psbB_p2	TCAACCCAAGTCGCTTCTTC	TATGCACCGAAAGCAATCGA	50.0	45.0	58.1	58.0	628-629 bp	1.0	79.3
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	TATGCACCGAAAGCAATCGA	50.0	45.0	57.8	58.0	585-586 bp	1.0	78.9
clpP-psbB_p4	TTTGGAACACCAATGGGCAT	TATGCACCGAAAGCAATCGA	45.0	45.0	58.3	58.0	583-584 bp	1.0	78.1
clpP-psbB_p5	AAGTCGCTTCTTCATCTCCG	TATGCACCGAAAGCAATCGA	50.0	45.0	57.7	58.0	621-622 bp	1.0	78.0