Hotspot

trnT-trnL

LSC · 47702-48527 · 826 bp

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Pi 0.00408
MarkerSeek score 50.00
Primer available yes
Label trnT-trnL

Feature Metadata

label_name
trnT-trnL
feature_type
igs
region
LSC
length_bp
826
variable_sites
5
pi
0.00408
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-trnL_p1 ATTTGAACCGATGACCCTCG ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.0 58.0 919-927 bp 1.0 81.0
trnT-trnL_p2 CGATGACCCTCGCATTACAA ACAATCAAGTCCGTAGCGTC 50.0 50.0 58.1 58.0 911-919 bp 1.0 80.7
trnT-trnL_p3 GCGATGCTCTAACCTCTGAG ACAATCAAGTCCGTAGCGTC 55.0 50.0 57.9 58.0 889-897 bp 1.0 80.2
trnT-trnL_p4 ATTTGAACCGATGACCCTCG CCGTAGCGTCTACCGATTTC 50.0 55.0 58.0 58.2 909-917 bp 1.0 79.1
trnT-trnL_p5 ATTTGAACCGATGACCCTCG CAATCAAGTCCGTAGCGTCT 50.0 50.0 58.0 57.7 918-926 bp 1.0 78.9