Hotspot

rps16-trnQ

LSC · 5610-7134 · 1525 bp

Back to Sasamorpha

Pi 0.00306

MarkerSeek score 60.60

Primer available yes

Label rps16-trnQ

Feature Metadata

label_name: rps16-trnQ
feature_type: igs
region: LSC
length_bp: 1525
variable_sites: 7
pi: 0.00306
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1599-1607 bp	1.0	78.6
rps16-trnQ_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1610-1618 bp	1.0	78.1
rps16-trnQ_p3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1603-1611 bp	1.0	77.5
rps16-trnQ_p4	TCATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	50.0	55.0	58.2	57.8	1614-1622 bp	1.0	77.0
rps16-trnQ_p5	AAGTCGCACGTTGCTTTCTA	TAAAACCAGAAATGGGGCGT	45.0	45.0	58.1	57.7	1659-1667 bp	1.0	76.9