Hotspot

rps3

LSC · 81338-82057 · 720 bp

Back to Sasaella

Pi 0.00833
MarkerSeek score 63.50
Primer available yes
Label rps3

Feature Metadata

label_name
rps3
feature_type
gene
region
LSC
length_bp
720
variable_sites
6
pi
0.00833
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3_p1 ACAGAATGGGAAGACAAGGC TCCATCTTAGATTTCGATTCCCA 50.0 39.1 57.8 57.1 791-792 bp 1.0 48.0
rps3_p2 ACAGAATGGGAAGACAAGGC TCCATCTTAGATTTCGATTCCCAG 50.0 41.7 57.8 58.1 791-792 bp 1.0 45.9
rps3_p3 CAGAATGGGAAGACAAGGCT TCCATCTTAGATTTCGATTCCCA 50.0 39.1 57.5 57.1 790-791 bp 1.0 45.6
rps3_p4 ACAGAATGGGAAGACAAGGC CCATCTTAGATTTCGATTCCCAG 50.0 43.5 57.8 56.8 790-791 bp 1.0 45.5
rps3_p5 ACAGAATGGGAAGACAAGGC CCATCTTAGATTTCGATTCCCA 50.0 40.9 57.8 55.7 790-791 bp 1.0 44.4