Hotspot

psbB

LSC · 70725-72251 · 1527 bp

Back to Sasaella

Pi 0.00196

MarkerSeek score 50.40

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 3
pi: 0.00196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	TGACACAGAATCCCCTAGAGG	AGAAAGGATGGGAGATGTTTCC	52.4	45.5	58.2	57.8	1708 bp	1.0	52.2
psbB_p2	GACACAGAATCCCCTAGAGG	AGAAAGGATGGGAGATGTTTCC	55.0	45.5	56.4	57.8	1707 bp	1.0	49.5
psbB_p3	TGACACAGAATCCCCTAGAGG	GGAGATGTTTCCCATGTCAA	52.4	45.0	58.2	55.3	1698 bp	1.0	48.7
psbB_p4	TGACACAGAATCCCCTAGAGG	GAAAGGATGGGAGATGTTTCC	52.4	47.6	58.2	56.3	1707 bp	1.0	48.4
psbB_p5	TCTGACACAGAATCCCCTAGA	AGAAAGGATGGGAGATGTTTCC	47.6	45.5	57.2	57.8	1710 bp	1.0	48.1