Hotspot

atpB

LSC · 54026-55522 · 1497 bp

Back to Sasaella

Pi 0.00401

MarkerSeek score 55.70

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1497
variable_sites: 6
pi: 0.00401
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	CGTGGTTTGGTAATACGCCA	TGACTTTTGGATTTTTGGCT	50.0	35.0	58.6	53.8	1706-1707 bp	1.0	42.5
atpB_p2	CGTGGTTTGGTAATACGCCA	CCTTTTGACTTTTGGATTTTTGGC	50.0	37.5	58.6	58.2	1711-1712 bp	1.0	42.4
atpB_p3	CGTGGTTTGGTAATACGCCA	TTGACTTTTGGATTTTTGGCT	50.0	33.3	58.6	54.7	1707-1708 bp	1.0	41.6
atpB_p4	CGTGGTTTGGTAATACGCCA	TTTGACTTTTGGATTTTTGGCT	50.0	31.8	58.6	55.5	1708-1709 bp	1.0	40.3
atpB_p5	GGGGCGTGGTTTGGTAATAC	TGACTTTTGGATTTTTGGCT	55.0	35.0	58.9	53.8	1710-1711 bp	1.0	40.3