Hotspot

ndhG-ndhI

SSC · 113278-113523 · 246 bp

Back to Sasaella

Pi 0.0366

MarkerSeek score 62.40

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 246
variable_sites: 9
pi: 0.0366
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCCCCAAATAGCACAAGAA	GAGTCGGTTACCAATCTCCA	45.0	50.0	56.7	56.7	412-428 bp	1.0	60.7
ndhG-ndhI_p2	TCCCCCAAATAGCACAAGAA	TGAGTCGGTTACCAATCTCC	45.0	50.0	56.7	56.7	413-429 bp	1.0	60.7
ndhG-ndhI_p3	TCCCCCAAATAGCACAAGAA	TGAGTCGGTTACCAATCTCCA	45.0	47.6	56.7	58.5	413-429 bp	1.0	59.7
ndhG-ndhI_p4	ATCCCCCAAATAGCACAAGA	GAGTCGGTTACCAATCTCCA	45.0	50.0	56.5	56.7	413-429 bp	1.0	58.8
ndhG-ndhI_p5	ATCCCCCAAATAGCACAAGA	TGAGTCGGTTACCAATCTCC	45.0	50.0	56.5	56.7	414-430 bp	1.0	58.8