Hotspot

petA-psbJ

LSC · 62346-63340 · 995 bp

Back to Sasaella

Pi 0.00407

MarkerSeek score 61.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 995
variable_sites: 4
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTCGC	TCCTCTTTGGCTGATAGGTACT	50.0	45.5	57.9	58.0	1186-1197 bp	1.0	64.6
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	CCTCTTTGGCTGATAGGTACTG	50.0	50.0	57.9	57.9	1185-1196 bp	1.0	64.1
petA-psbJ_p3	GGCCTTTTGTTCTTCTTCGC	TTCCTCTTTGGCTGATAGGT	50.0	45.0	57.9	55.8	1187-1198 bp	1.0	63.2
petA-psbJ_p4	GGCCTTTTGTTCTTCTTCGC	ACGGTTCATATTCTGGATTGGG	50.0	45.5	57.9	58.2	1121-1132 bp	1.0	63.2
petA-psbJ_p5	GGCCTTTTGTTCTTCTTCGC	CGGTTCATATTCTGGATTGGGT	50.0	45.5	57.9	58.2	1120-1131 bp	1.0	63.2