Hotspot

accD-psaI

LSC · 58360-59027 · 668 bp

Back to Sasaella

Pi 0.00599

MarkerSeek score 62.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 4
pi: 0.00599
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCCTTTACCGCGCATTATTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	763-768 bp	1.0	78.2
accD-psaI_p2	GCCTTTACCGCGCATTATTG	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.2	57.9	769-774 bp	1.0	77.6
accD-psaI_p3	CTATTTTTCCGCCTTTACCGC	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.9	58.0	773-778 bp	1.0	71.7
accD-psaI_p4	GCTATTTTTCCGCCTTTACCG	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.9	58.0	774-779 bp	1.0	71.7
accD-psaI_p5	CCTTTACCGCGCATTATTGT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.9	58.0	762-767 bp	1.0	71.2