Hotspot

rpoC2-rps2

LSC · 31055-31356 · 302 bp

Back to Sasaella

Pi 0.0133

MarkerSeek score 59.90

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 302
variable_sites: 4
pi: 0.0133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	ATGAGACATCAGAACCCCCA	ACCAAAATGAACTCCCGCTT	50.0	45.0	58.0	58.0	408-409 bp	1.0	80.9
rpoC2-rps2_p2	TGAGACATCAGAACCCCCAT	ACCAAAATGAACTCCCGCTT	50.0	45.0	58.0	58.0	407-408 bp	1.0	80.9
rpoC2-rps2_p3	ATGAGACATCAGAACCCCCA	TTTACGCTTTGCCGAGATGT	50.0	45.0	58.0	58.2	465-466 bp	1.0	79.2
rpoC2-rps2_p4	TGAGACATCAGAACCCCCAT	TTTACGCTTTGCCGAGATGT	50.0	45.0	58.0	58.2	464-465 bp	1.0	79.2
rpoC2-rps2_p5	TGAGACATCAGAACCCCCAT	CTTTGCCGAGATGTAAGGGG	50.0	55.0	58.0	58.3	458-459 bp	1.0	78.2