Hotspot

trnC-GCA-rpoB

LSC · 19831-20990 · 1160 bp

Back to Sasaella

Pi 0.00431

MarkerSeek score 52.80

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1160
variable_sites: 5
pi: 0.00431
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1244-1262 bp	1.0	77.2
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1243-1261 bp	1.0	77.2
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGCCTT	50.0	45.0	58.1	57.6	1308-1326 bp	1.0	76.8
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	AGTTCTTCTGCCAAGCCTTG	50.0	50.0	58.1	58.4	1307-1325 bp	1.0	76.6
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	CAAGTTCTTCTGCCAAGCCT	50.0	50.0	58.1	58.4	1309-1327 bp	1.0	76.6