Hotspot

psbM-petN

LSC · 18000-18771 · 772 bp

Back to Sasaella

Pi 0.00526

MarkerSeek score 60.60

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 4
pi: 0.00526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGGAAGTCAATATTCTCGCA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	54.5	58.2	947-959 bp	1.0	52.0
psbM-petN_p2	TGGAAGTCAATATTCTCGCA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	54.5	58.3	891-903 bp	1.0	51.0
psbM-petN_p3	TGGAAGTCAATATTCTCGCA	TAGTATGGGGGAGGAGTGGA	40.0	55.0	54.5	58.4	890-902 bp	1.0	50.3
psbM-petN_p4	TGCTACTGCACTGTTTATTCT	TAGTAAGTCTCGCTTGGGCT	38.1	50.0	55.2	58.2	922-934 bp	1.0	50.1
psbM-petN_p5	TGCTACTGCACTGTTTATTCT	GTAGTATGGGGGAGGAGTGG	38.1	60.0	55.2	58.3	866-878 bp	1.0	49.2