Hotspot

petN-trnC-GCA

LSC · 19551-20462 · 912 bp

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Pi 0.0022
MarkerSeek score 52.90
Primer available yes
Label petN-trnC-GCA

Feature Metadata

label_name
petN-trnC-GCA
feature_type
igs
region
LSC
length_bp
912
variable_sites
4
pi
0.0022
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-trnC-GCA_p1 AGCCCAAGCGAGACTTACTA AGTTCAAATCTGGGTGCCG 50.0 52.6 58.2 58.1 947-961 bp 1.0 71.9
petN-trnC-GCA_p2 AGCCCAAGCGAGACTTACTA CCAGTTCAAATCTGGGTGCC 50.0 55.0 58.2 59.1 949-963 bp 1.0 71.4
petN-trnC-GCA_p3 CCACTCCTCCCCCATACTAC AGTTCAAATCTGGGTGCCG 60.0 52.6 58.3 58.1 1003-1017 bp 1.0 70.7
petN-trnC-GCA_p4 CCACTCCTCCCCCATACTAC CCAGTTCAAATCTGGGTGCC 60.0 55.0 58.3 59.1 1005-1019 bp 1.0 70.3
petN-trnC-GCA_p5 TCCACTCCTCCCCCATACTA AGTTCAAATCTGGGTGCCG 55.0 52.6 58.4 58.1 1004-1018 bp 1.0 70.0