Hotspot

psbM-petN

LSC · 18701-19460 · 760 bp

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Pi 0.00351
MarkerSeek score 61.90
Primer available yes
Label psbM-petN

Feature Metadata

label_name
psbM-petN
feature_type
igs
region
LSC
length_bp
760
variable_sites
5
pi
0.00351
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-petN_p1 TGGAAGTCAATATTCTCGCA TAGTAAGTCTCGCTTGGGCT 40.0 50.0 54.5 58.2 941-947 bp 1.0 51.6
psbM-petN_p2 TGGAAGTCAATATTCTCGCA GTAGTATGGGGGAGGAGTGG 40.0 60.0 54.5 58.3 885-891 bp 1.0 50.6
psbM-petN_p3 TGGAAGTCAATATTCTCGCA TAGTATGGGGGAGGAGTGGA 40.0 55.0 54.5 58.4 884-890 bp 1.0 49.9
psbM-petN_p4 TGCTACTGCACTGTTTATTCT TAGTAAGTCTCGCTTGGGCT 38.1 50.0 55.2 58.2 916-922 bp 1.0 49.7
psbM-petN_p5 TGCTACTGCACTGTTTATTCT GTAGTATGGGGGAGGAGTGG 38.1 60.0 55.2 58.3 860-866 bp 1.0 48.7