Hotspot

trnG-UCC-trnT-GGU

LSC · 14401-16358 · 1958 bp

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Pi 0.00366
MarkerSeek score 60.30
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1958
variable_sites
14
pi
0.00366
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT CCATGGCATTACTCTACCACC 38.1 52.4 55.5 58.1 2093-2130 bp 1.0 43.1
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT GACTTACGTCTTACCATGGCA 38.1 47.6 55.5 57.8 2106-2143 bp 1.0 42.3
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT TGACTTACGTCTTACCATGGC 38.1 47.6 55.5 57.8 2107-2144 bp 1.0 42.3
trnG-UCC-trnT-GGU_p4 AGAACGAATCACACTTTTACCAC CCATGGCATTACTCTACCACC 39.1 52.4 57.3 58.1 2096-2133 bp 1.0 42.0
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT CCATGGCATTACTCTACCACC 39.1 52.4 57.3 58.1 2095-2132 bp 1.0 42.0