Hotspot

trnS-GUA-psbD

LSC · 8465-9465 · 1001 bp

Back to Sasa

Pi 0.000999
MarkerSeek score 61.80
Primer available yes
Label trnS-GUA-psbD

Feature Metadata

label_name
trnS-GUA-psbD
feature_type
igs
region
LSC
length_bp
1001
variable_sites
2
pi
0.000999
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GUA-psbD_p1 ATTAGCAATCCGCCGCTTTA GCCGGACCATCCTACAAAAA 45.0 50.0 58.0 58.2 1128-1166 bp 1.0 80.1
trnS-GUA-psbD_p2 ATTAGCAATCCGCCGCTTTA ACAAAAACGAAACGGTCCCT 45.0 45.0 58.0 58.2 1115-1153 bp 1.0 79.6
trnS-GUA-psbD_p3 GCTTTAGTCCACTCAGCCAT GCCGGACCATCCTACAAAAA 50.0 50.0 57.6 58.2 1114-1152 bp 1.0 77.2
trnS-GUA-psbD_p4 ATTAGCAATCCGCCGCTTTA GAAACGGTCCCTTCGTAACC 45.0 55.0 58.0 58.6 1107-1145 bp 1.0 77.0
trnS-GUA-psbD_p5 GCTTTAGTCCACTCAGCCAT ACAAAAACGAAACGGTCCCT 50.0 45.0 57.6 58.2 1101-1139 bp 1.0 76.6