Hotspot

rpl16

LSC · 77234-78712 · 1479 bp

Back to Sartidia

Pi 0.00766

MarkerSeek score 50.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1479
variable_sites: 17
pi: 0.00766
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCGTTGATTACCAGCAGCTC	AATTGCAGGTCGTCTAGCAG	50.0	50.0	58.0	58.0	2013-2017 bp	1.0	80.7
rpl16_p2	AATTGCGCCAAAAACTCGAG	AATTGCAGGTCGTCTAGCAG	45.0	50.0	57.9	58.0	2226-2230 bp	1.0	80.3
rpl16_p3	CTAGAGGCATTTGGGGCAAT	AATTGCAGGTCGTCTAGCAG	50.0	50.0	57.9	58.0	2077-2081 bp	1.0	80.1
rpl16_p4	TCAGCAATAGTGTCCTTGCC	AATTGCAGGTCGTCTAGCAG	50.0	50.0	57.9	58.0	2451-2455 bp	1.0	79.7
rpl16_p5	TTTCTAGAGGCATTTGGGGC	AATTGCAGGTCGTCTAGCAG	50.0	50.0	57.9	58.0	2080-2084 bp	1.0	79.6