Hotspot

rpl32-trnL-UAG

SSC · 105952-106440 · 489 bp

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Pi 0.0204

MarkerSeek score 61.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 489
variable_sites: 15
pi: 0.0204
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGCAGCAACGAGTATCCAAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.5	57.9	592-597 bp	1.0	77.9
rpl32-trnL-UAG_p2	AGCAGCAACGAGTATCCAAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	57.5	57.8	585-590 bp	1.0	77.5
rpl32-trnL-UAG_p3	AGCAGCAACGAGTATCCAAA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	57.5	58.2	606-611 bp	1.0	77.4
rpl32-trnL-UAG_p4	AGCAGCAACGAGTATCCAAA	CCTAAGAGCAGCGTGTCTAC	45.0	55.0	57.5	57.8	591-596 bp	1.0	77.3
rpl32-trnL-UAG_p5	AGCAGCAACGAGTATCCAAA	CTAAGAGCAGCGTGTCTACC	45.0	55.0	57.5	57.8	590-595 bp	1.0	77.3