Hotspot

rps12-clpP

LSC · 67131-67270 · 140 bp

Back to Sartidia

Pi 0.019

MarkerSeek score 60.90

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 140
variable_sites: 4
pi: 0.019
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CACCCTAGCACATGTTCCTC	ACTGCGATACTGATCCGGTA	55.0	50.0	58.0	58.0	301 bp	1.0	80.6
rps12-clpP_p2	CCTAGCACATGTTCCTCGAC	ACTGCGATACTGATCCGGTA	55.0	50.0	58.1	58.0	298 bp	1.0	80.3
rps12-clpP_p3	TTCGTATTGGCTGTCTTGCA	ACTGCGATACTGATCCGGTA	45.0	50.0	57.8	58.0	239 bp	1.0	79.7
rps12-clpP_p4	CACCCTAGCACATGTTCCTC	GATTGACGAGCACTGCGATA	55.0	50.0	58.0	58.2	312 bp	1.0	79.5
rps12-clpP_p5	CCTAGCACATGTTCCTCGAC	GATTGACGAGCACTGCGATA	55.0	50.0	58.1	58.2	309 bp	1.0	79.2