Hotspot

trnP-UGG-psaJ

LSC · 63718-64105 · 388 bp

Back to Sartidia

Pi 0.0137

MarkerSeek score 61.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 388
variable_sites: 8
pi: 0.0137
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	AAAACAAACGCGCTACCAAG	CGACAAAGCATCCGGGAATA	45.0	50.0	57.9	58.1	537 bp	1.0	79.9
trnP-UGG-psaJ_p2	AAAACAAACGCGCTACCAAG	ACCTGCTAAAGCTCCAAACC	45.0	50.0	57.9	58.1	495 bp	1.0	79.7
trnP-UGG-psaJ_p3	AAAACAAACGCGCTACCAAG	CCTGCTAAAGCTCCAAACCA	45.0	50.0	57.9	58.1	494 bp	1.0	79.7
trnP-UGG-psaJ_p4	CCCCGCTACTGCTCATAAAG	CGACAAAGCATCCGGGAATA	55.0	50.0	58.1	58.1	613 bp	1.0	79.6
trnP-UGG-psaJ_p5	CCCCGCTACTGCTCATAAAG	ACCTGCTAAAGCTCCAAACC	55.0	50.0	58.1	58.1	571 bp	1.0	79.4