Hotspot

rbcL-psaI

LSC · 56044-56961 · 918 bp

Back to Sartidia

Pi 0.0122

MarkerSeek score 55.30

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 918
variable_sites: 15
pi: 0.0122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TACAAGCTCGTAACGAAGGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1121-1137 bp	1.0	82.5
rbcL-psaI_p2	GGCTTCAGGGGGTATTCATG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1273-1289 bp	1.0	82.2
rbcL-psaI_p3	TACAAGCTCGTAACGAAGGC	TCGCTATACTCGGTTAGGCA	50.0	50.0	58.0	58.0	1342-1353 bp	1.0	82.1
rbcL-psaI_p4	GGCTTCAGGGGGTATTCATG	TCGCTATACTCGGTTAGGCA	55.0	50.0	58.0	58.0	1494-1505 bp	1.0	81.9
rbcL-psaI_p5	GGCTTCAGGGGGTATTCATG	AATCGGTCATAACCACTGCC	55.0	50.0	58.0	58.0	1955-1966 bp	1.0	81.4