Hotspot

ndhC-trnV-UAC

LSC · 49954-50861 · 908 bp

Back to Sartidia

Pi 0.00442
MarkerSeek score 63.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
908
variable_sites
6
pi
0.00442
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CGGAGCTAAAAGCCCTGAAA GGTTCGAATCCGTATAGCCC 50.0 55.0 58.2 57.9 1018-1029 bp 1.0 79.6
ndhC-trnV-UAC_p2 CGGAGCTAAAAGCCCTGAAA AGCCCAATGCAGAAAAATGC 50.0 45.0 58.2 57.9 1111-1122 bp 1.0 79.2
ndhC-trnV-UAC_p3 CTAACCGGAGCTAAAAGCCC GGTTCGAATCCGTATAGCCC 55.0 55.0 58.3 57.9 1023-1034 bp 1.0 78.3
ndhC-trnV-UAC_p4 CTAACCGGAGCTAAAAGCCC AGCCCAATGCAGAAAAATGC 55.0 45.0 58.3 57.9 1116-1127 bp 1.0 78.0
ndhC-trnV-UAC_p5 ACTAACCGGAGCTAAAAGCC GGTTCGAATCCGTATAGCCC 50.0 55.0 57.6 57.9 1024-1035 bp 1.0 77.8