Hotspot

atpI-atpH

LSC · 31377-32206 · 830 bp

Back to Sartidia

Pi 0.00402

MarkerSeek score 61.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 830
variable_sites: 5
pi: 0.00402
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	TAGCCGCAGCCTATATAGGT	AGCCAATCCAGCAGCAATAA	50.0	45.0	57.5	57.9	920-923 bp	1.0	75.4
atpI-atpH_p2	TAGCCGCAGCCTATATAGGT	GCAGTACCTTGACCAACTCC	50.0	55.0	57.5	58.2	964-967 bp	1.0	75.0
atpI-atpH_p3	TAGCCGCAGCCTATATAGGT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	57.5	57.8	897-900 bp	1.0	74.9
atpI-atpH_p4	ATTTTTGCAACGTTAGCCGC	AGCCAATCCAGCAGCAATAA	45.0	45.0	58.6	57.9	933-936 bp	1.0	74.9
atpI-atpH_p5	TAGCCGCAGCCTATATAGGT	GCCAATCCAGCAGCAATAAC	50.0	50.0	57.5	57.8	919-922 bp	1.0	74.9