Hotspot

petN-trnC-GCA

LSC · 17237-18174 · 938 bp

Back to Sartidia

Pi 0.00789

MarkerSeek score 61.80

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 938
variable_sites: 11
pi: 0.00789
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	977-987 bp	1.0	72.1
petN-trnC-GCA_p2	AGCCCAAGCGAGACTTACTA	CCAGTTCAAATCTGGGTGCC	50.0	55.0	58.2	59.1	979-989 bp	1.0	71.6
petN-trnC-GCA_p3	CCACTCCTCCCCCATACTAC	AGTTCAAATCTGGGTGCCG	60.0	52.6	58.3	58.1	1033-1043 bp	1.0	70.9
petN-trnC-GCA_p4	CCACTCCTCCCCCATACTAC	CCAGTTCAAATCTGGGTGCC	60.0	55.0	58.3	59.1	1035-1045 bp	1.0	70.5
petN-trnC-GCA_p5	TCCACTCCTCCCCCATACTA	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.4	58.1	1034-1044 bp	1.0	70.2