Hotspot

trnT-GGU-trnE-UUC

LSC · 14798-15332 · 535 bp

Back to Sartidia

Pi 0.0138

MarkerSeek score 55.30

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 535
variable_sites: 11
pi: 0.0138
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	AGCCCTTTTAACTCAGTGGT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.7	57.7	631-635 bp	1.0	68.9
trnT-GGU-trnE-UUC_p2	AGGCATAAGTCATCGGTTCA	TGTCCTGAACCACTAGACGA	45.0	50.0	56.6	57.7	594-598 bp	1.0	68.8
trnT-GGU-trnE-UUC_p3	TGGTAAGGCATAAGTCATCGG	TGTCCTGAACCACTAGACGA	47.6	50.0	57.5	57.7	599-603 bp	1.0	68.0
trnT-GGU-trnE-UUC_p4	GGTAAGGCATAAGTCATCGGT	TGTCCTGAACCACTAGACGA	47.6	50.0	57.5	57.7	598-602 bp	1.0	68.0
trnT-GGU-trnE-UUC_p5	GTGGTAGAGTAATGCCATGGT	TGTCCTGAACCACTAGACGA	47.6	50.0	57.4	57.7	616-620 bp	1.0	67.2