Hotspot

trnG-GCC-trnfM-CAU

LSC · 12232-12596 · 365 bp

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Pi 0.011

MarkerSeek score 54.30

Primer available yes

Label trnG-GCC-trnfM-CAU

Feature Metadata

label_name: trnG-GCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 365
variable_sites: 6
pi: 0.011
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnfM-CAU_p1	GTGTAGCGAGCGTAGTTCAA	ACCTGTTTTTGGCCCTACAG	50.0	50.0	58.0	58.0	531-535 bp	1.0	81.4
trnG-GCC-trnfM-CAU_p2	TGTAGCGAGCGTAGTTCAAC	ACCTGTTTTTGGCCCTACAG	50.0	50.0	58.0	58.0	530-534 bp	1.0	81.4
trnG-GCC-trnfM-CAU_p3	TGTAGCGAGCGTAGTTCAAC	GACCTGTTTTTGGCCCTACA	50.0	50.0	58.0	58.0	531-535 bp	1.0	81.4
trnG-GCC-trnfM-CAU_p4	GTGTAGCGAGCGTAGTTCAA	GACCTGTTTTTGGCCCTACA	50.0	50.0	58.0	58.0	532-536 bp	1.0	81.3
trnG-GCC-trnfM-CAU_p5	GAAGGTACGGGTTCGATTCC	ACCTGTTTTTGGCCCTACAG	55.0	50.0	58.1	58.0	486-490 bp	1.0	81.2