Hotspot

ycf1

IRb · 112394-118066 · 5673 bp

Back to Sarracenia

Pi 0.0625

MarkerSeek score 60.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 5673
variable_sites: 203
pi: 0.0625
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.57
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATCCTCCTTGTCACAAGCAT	TTCTTGGATTTCGCAGTCGA	45.0	45.0	56.8	57.6	1409 bp	0.5	46.9
ycf1_p2	ATCCTCCTTGTCACAAGCAT	TGCTTCCGTATTGCTCTTGT	45.0	45.0	56.8	57.5	1285 bp	0.5	46.7
ycf1_p3	ATCCTCCTTGTCACAAGCAT	GCTCTCGGCATCCTTGATTA	45.0	50.0	56.8	57.5	1628 bp	0.5	46.2
ycf1_p4	ATCCTCCTTGTCACAAGCAT	AGTTCTGCTTCCGTATTGCT	45.0	45.0	56.8	57.2	1290 bp	0.5	44.3
ycf1_p5	ACGAAAATAAGAGGAACCCGT	TTCTTGGATTTCGCAGTCGA	42.9	45.0	57.3	57.6	948 bp	0.5	42.9