Hotspot

rps15

IRb · 118441-118677 · 237 bp

Back to Sarracenia

Pi 0.419

MarkerSeek score 65.90

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: IRb
length_bp: 237
variable_sites: 39
pi: 0.419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.39
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	TGGCAGTCGTTTCAGTATGT	ATGGGTTACCGCCACAAATT	45.0	45.0	57.2	58.1	1271 bp	0.5	52.8
rps15_p2	TGGCAGTCGTTTCAGTATGT	CTATGGGTTACCGCCACAAA	45.0	50.0	57.2	57.9	1273 bp	0.5	52.4
rps15_p3	TGGCAGTCGTTTCAGTATGT	CTCTCACACTCTGCTAGGGA	45.0	55.0	57.2	57.9	1316 bp	0.5	52.3
rps15_p4	TGGCAGTCGTTTCAGTATGT	TCTCTCACACTCTGCTAGGG	45.0	55.0	57.2	57.9	1317 bp	0.5	52.3
rps15_p5	TGGCAGTCGTTTCAGTATGT	TACCGCCACAAATTCCATCC	45.0	50.0	57.2	58.2	1265 bp	0.5	51.4