Hotspot

ndhI

IRb · 119638-119952 · 315 bp

Back to Sarracenia

Pi 0.344

MarkerSeek score 63.70

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: IRb
length_bp: 315
variable_sites: 22
pi: 0.344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.20
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	AGACTTTACCAAAGCTGAGA	TGTAGATCAAAACTGAACCCT	40.0	38.1	53.9	54.5	399 bp	0.5	20.0
ndhI_p2	AGACTTTACCAAAGCTGAGA	TGTAGATCAAAACTGAACCCTAGT	40.0	37.5	53.9	57.3	399 bp	0.5	20.0
ndhI_p3	AGACTTTACCAAAGCTGAGA	AGATCAAAACTGAACCCTAGT	40.0	38.1	53.9	54.2	396 bp	0.5	20.0
ndhI_p4	AGACTTTACCAAAGCTGAGA	ATGTAGATCAAAACTGAACCCT	40.0	36.4	53.9	55.1	400 bp	0.5	20.0
ndhI_p5	AGACTTTACCAAAGCTGAGA	TGTAGATCAAAACTGAACCC	40.0	40.0	53.9	52.8	399 bp	0.5	20.0