Hotspot

atpI

LSC · 15386-16129 · 744 bp

Back to Sarracenia

Pi 0.00134

MarkerSeek score 59.50

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 744
variable_sites: 1
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	GGCTTGGGCTCGACTAAAAA	CGATTCAAGTAGGCAAGTCGA	50.0	47.6	58.5	58.1	874 bp	1.0	67.3
atpI_p2	GGCTTGGGCTCGACTAAAAA	GGCAAGTCGAGAAAGAGAAGA	50.0	47.6	58.5	57.7	863 bp	1.0	65.9
atpI_p3	GGCTTGGGCTCGACTAAAAA	AGGCAAGTCGAGAAAGAGAAG	50.0	47.6	58.5	57.7	864 bp	1.0	65.8
atpI_p4	GGCTTGGGCTCGACTAAAAA	CGATTCAAGTAGGCAAGTCG	50.0	50.0	58.5	56.6	874 bp	1.0	65.5
atpI_p5	GGCTTGGGCTCGACTAAAAA	ACGATTCAAGTAGGCAAGTCG	50.0	47.6	58.5	58.4	875 bp	1.0	65.2