Hotspot

ndhI-rpl32

IRb · 119953-120964 · 1012 bp

Back to Sarracenia

Pi 0.166

MarkerSeek score 70.20

Primer available yes

Label ndhI-rpl32

Feature Metadata

label_name: ndhI-rpl32
feature_type: igs
region: IRb
length_bp: 1012
variable_sites: 53
pi: 0.166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.32
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-rpl32_p1	TGACGATTCCACAATTCGAA	AGCTTTTAAGGCTGCCCAAT	40.0	45.0	55.4	58.1	1154 bp	0.5	38.8
ndhI-rpl32_p2	TTGACGATTCCACAATTCGA	AGCTTTTAAGGCTGCCCAAT	40.0	45.0	55.4	58.1	1155 bp	0.5	38.8
ndhI-rpl32_p3	GTCGTTTACCAATGTCAGGA	AGCTTTTAAGGCTGCCCAAT	45.0	45.0	55.4	58.1	1176 bp	0.5	38.8
ndhI-rpl32_p4	TTGACGATTCCACAATTCGAA	AGCTTTTAAGGCTGCCCAAT	38.1	45.0	56.2	58.1	1155 bp	0.5	37.1
ndhI-rpl32_p5	GTCGTTTACCAATGTCAGGAA	AGCTTTTAAGGCTGCCCAAT	42.9	45.0	56.2	58.1	1176 bp	0.5	37.1