Hotspot

rps15-ndhI

IRb · 118678-119637 · 960 bp

Back to Sarracenia

Pi 0.36
MarkerSeek score 70.80
Primer available yes
Label rps15-ndhI

Feature Metadata

label_name
rps15-ndhI
feature_type
igs
region
IRb
length_bp
960
variable_sites
98
pi
0.36
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.28
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ndhI_p1 GTCAACGCCTGCTAGCTTAT TGCTGACTTGACCAGGAAAA 50.0 45.0 58.1 57.3 1691 bp 0.5 53.7
rps15-ndhI_p2 GTCAACGCCTGCTAGCTTAT TTGCTGACTTGACCAGGAAA 50.0 45.0 58.1 57.3 1692 bp 0.5 53.7
rps15-ndhI_p3 GTCAACGCCTGCTAGCTTAT TTTGCTGACTTGACCAGGAA 50.0 45.0 58.1 57.3 1693 bp 0.5 53.7
rps15-ndhI_p4 GTCAACGCCTGCTAGCTTAT TTTTGCTGACTTGACCAGGA 50.0 45.0 58.1 57.3 1694 bp 0.5 53.7
rps15-ndhI_p5 GTCAACGCCTGCTAGCTTAT TCGTGATTCTCACCAACCAA 50.0 45.0 58.1 57.1 1471 bp 0.5 52.2