Hotspot

rpoB-trnC-GCA

LSC · 27686-28921 · 1236 bp

Back to Sarracenia

Pi 0.000809

MarkerSeek score 59.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1236
variable_sites: 1
pi: 0.000809
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCAAGTTCTTCCGTCAAGCC	CTTGACTTGATCAGGCGACA	50.0	50.0	58.1	57.9	1426 bp	1.0	78.4
rpoB-trnC-GCA_p2	TCAAGTTCTTCCGTCAAGCC	TTGACTTGATCAGGCGACAC	50.0	50.0	58.1	58.2	1425 bp	1.0	77.5
rpoB-trnC-GCA_p3	GTCAAGCCCCGATCAATGAA	CTTGACTTGATCAGGCGACA	50.0	50.0	58.2	57.9	1414 bp	1.0	77.4
rpoB-trnC-GCA_p4	TCAAGCCCCGATCAATGAAC	CTTGACTTGATCAGGCGACA	50.0	50.0	58.2	57.9	1413 bp	1.0	77.4
rpoB-trnC-GCA_p5	TCAAGCCCCGATCAATGAAC	TTGACTTGATCAGGCGACAC	50.0	50.0	58.2	58.2	1412 bp	1.0	76.4