primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC_p1	trnG-UCC	1	CCAATTCCCCTGTTCGACAA	AGAGTATAGAAGAGAGGCTCT	20	21	50.0	42.9	58.1	54.1	0.0	18.8	5.034209	9623	10382	835-835	1.000000	40.1
trnG-UCC_p2	trnG-UCC	2	GCACACTCGCATTTTCATGA	AGAGTATAGAAGAGAGGCTCT	20	21	45.0	42.9	57.4	54.1	0.0	18.8	5.555746	9623	10382	883-883	1.000000	40.1
trnG-UCC_p3	trnG-UCC	3	TCGATTACACCCAATTCCCC	AGAGTATAGAAGAGAGGCTCT	20	21	50.0	42.9	57.3	54.1	0.0	18.8	5.659921	9623	10382	845-845	1.000000	40.1
trnG-UCC_p4	trnG-UCC	4	CGATTACACCCAATTCCCCT	AGAGTATAGAAGAGAGGCTCT	20	21	50.0	42.9	57.3	54.1	0.0	18.8	5.667523	9623	10382	844-844	1.000000	40.1
trnG-UCC_p5	trnG-UCC	5	CGCACACTCGCATTTTCATG	AGAGTATAGAAGAGAGGCTCT	20	21	50.0	42.9	58.7	54.1	0.0	18.8	5.685379	9623	10382	884-884	1.000000	40.1
atpI_p1	atpI	1	GGCTTGGGCTCGACTAAAAA	CGATTCAAGTAGGCAAGTCGA	20	21	50.0	47.6	58.5	58.1	0.0	0.0	1.593585	15386	16129	874-874	1.000000	67.3
atpI_p2	atpI	2	GGCTTGGGCTCGACTAAAAA	GGCAAGTCGAGAAAGAGAAGA	20	21	50.0	47.6	58.5	57.7	0.0	0.0	1.775075	15386	16129	863-863	1.000000	65.9
atpI_p3	atpI	3	GGCTTGGGCTCGACTAAAAA	AGGCAAGTCGAGAAAGAGAAG	20	21	50.0	47.6	58.5	57.7	0.0	0.0	1.781162	15386	16129	864-864	1.000000	65.8
atpI_p4	atpI	4	GGCTTGGGCTCGACTAAAAA	CGATTCAAGTAGGCAAGTCG	20	20	50.0	50.0	58.5	56.6	0.0	0.0	1.823293	15386	16129	874-874	1.000000	65.5
atpI_p5	atpI	5	GGCTTGGGCTCGACTAAAAA	ACGATTCAAGTAGGCAAGTCG	20	21	50.0	47.6	58.5	58.4	0.0	0.0	1.862489	15386	16129	875-875	1.000000	65.2
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCAAGTTCTTCCGTCAAGCC	CTTGACTTGATCAGGCGACA	20	20	50.0	50.0	58.1	57.9	0.0	4.5	0.205656	27686	28921	1426-1426	1.000000	78.4
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TCAAGTTCTTCCGTCAAGCC	TTGACTTGATCAGGCGACAC	20	20	50.0	50.0	58.1	58.2	0.0	4.5	0.325279	27686	28921	1425-1425	1.000000	77.5
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	GTCAAGCCCCGATCAATGAA	CTTGACTTGATCAGGCGACA	20	20	50.0	50.0	58.2	57.9	0.0	4.5	0.334417	27686	28921	1414-1414	1.000000	77.4
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TCAAGCCCCGATCAATGAAC	CTTGACTTGATCAGGCGACA	20	20	50.0	50.0	58.2	57.9	0.0	4.5	0.334417	27686	28921	1413-1413	1.000000	77.4
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCAAGCCCCGATCAATGAAC	TTGACTTGATCAGGCGACAC	20	20	50.0	50.0	58.2	58.2	0.0	4.5	0.454040	27686	28921	1412-1412	1.000000	76.4
petN-psbM_p1	petN-psbM	1	AGTAAATCTCGCTTGGGCTG	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	58.0	57.6	0.0	0.0	1.437262	29881	30908	1189-1189	1.000000	68.6
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	20	21	50.0	42.9	57.6	57.6	0.0	0.0	1.758286	29881	30908	1129-1129	1.000000	66.0
petN-psbM_p3	petN-psbM	3	AGTAAATCTCGCTTGGGCTG	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	58.0	56.1	0.0	0.0	1.959810	29881	30908	1189-1189	1.000000	64.4
petN-psbM_p4	petN-psbM	4	AGTAAATCTCGCTTGGGCTG	GCTACTGCACTGTTCATTCT	20	20	50.0	45.0	58.0	55.8	0.0	0.0	2.259137	29881	30908	1188-1188	1.000000	62.0
petN-psbM_p5	petN-psbM	5	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTC	20	20	50.0	45.0	57.6	56.1	0.0	0.0	2.280835	29881	30908	1129-1129	1.000000	61.8
accD-psaI_p1	accD-psaI	1	GCTCCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.8	58.0	0.0	26.9	0.787656	61255	61850	695-700	1.000000	74.3
accD-psaI_p2	accD-psaI	2	GCTCCACGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.8	57.9	0.0	26.8	0.864190	61255	61850	701-706	1.000000	73.7
accD-psaI_p3	accD-psaI	3	GCTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	19	20	52.6	45.0	58.1	58.0	0.0	26.9	1.092920	61255	61850	695-700	1.000000	71.8
accD-psaI_p4	accD-psaI	4	TCCACGCTTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	21	20	42.9	45.0	58.1	58.0	0.0	26.9	1.101279	61255	61850	693-698	1.000000	71.8
accD-psaI_p5	accD-psaI	5	GAGTTATTTCAGCTCCACGC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	56.9	58.0	0.0	26.9	1.122618	61255	61850	706-711	1.000000	71.6
ycf1_p1	ycf1	1	ATCCTCCTTGTCACAAGCAT	TTCTTGGATTTCGCAGTCGA	20	20	45.0	45.0	56.8	57.6	12.5	0.0	1.638428	116748	116997	1409-1409	0.500000	46.9
ycf1_p2	ycf1	2	ATCCTCCTTGTCACAAGCAT	TGCTTCCGTATTGCTCTTGT	20	20	45.0	45.0	56.8	57.5	12.5	0.0	1.662198	116748	116997	1285-1285	0.500000	46.7
ycf1_p3	ycf1	3	ATCCTCCTTGTCACAAGCAT	GCTCTCGGCATCCTTGATTA	20	20	45.0	50.0	56.8	57.5	12.5	0.0	1.722768	116748	116997	1628-1628	0.500000	46.2
ycf1_p4	ycf1	4	ATCCTCCTTGTCACAAGCAT	AGTTCTGCTTCCGTATTGCT	20	20	45.0	45.0	56.8	57.2	12.5	0.0	1.959666	116748	116997	1290-1290	0.500000	44.3
ycf1_p5	ycf1	5	ACGAAAATAAGAGGAACCCGT	TTCTTGGATTTCGCAGTCGA	21	20	42.9	45.0	57.3	57.6	0.0	0.0	2.139400	116748	116997	948-948	0.500000	42.9
ycf1-rps15_p1	ycf1-rps15	1	TCGACTGCGAAATCCAAGAA	ATAAGCTAGCAGGCGTTGAC	20	20	45.0	50.0	57.6	58.1	0.0	18.8	0.507601	118067	118440	1221-1221	0.500000	55.9
ycf1-rps15_p2	ycf1-rps15	2	TAATCAAGGATGCCGAGAGC	ATAAGCTAGCAGGCGTTGAC	20	20	50.0	50.0	57.5	58.1	0.0	18.8	0.591941	118067	118440	1002-1002	0.500000	55.3
ycf1-rps15_p3	ycf1-rps15	3	TCGACTGCGAAATCCAAGAA	ACAAATAAGCTAGCAGGCGT	20	20	45.0	45.0	57.6	57.6	0.0	18.8	0.852177	118067	118440	1225-1225	0.500000	53.2
ycf1-rps15_p4	ycf1-rps15	4	TCGACTGCGAAATCCAAGAA	CGGCTTCTTGAAGGTCACAA	20	20	45.0	50.0	57.6	58.4	0.0	11.7	0.855729	118067	118440	1707-1707	0.500000	53.2
ycf1-rps15_p5	ycf1-rps15	5	TGGCAGTCGTTTCAGTATGT	ATAAGCTAGCAGGCGTTGAC	20	20	45.0	50.0	57.2	58.1	0.0	18.8	0.890360	118067	118440	630-630	0.500000	52.9
rps15_p1	rps15	1	TGGCAGTCGTTTCAGTATGT	ATGGGTTACCGCCACAAATT	20	20	45.0	45.0	57.2	58.1	0.0	0.0	0.900050	118441	118677	1271-1271	0.500000	52.8
rps15_p2	rps15	2	TGGCAGTCGTTTCAGTATGT	CTATGGGTTACCGCCACAAA	20	20	45.0	50.0	57.2	57.9	0.0	0.0	0.948512	118441	118677	1273-1273	0.500000	52.4
rps15_p3	rps15	3	TGGCAGTCGTTTCAGTATGT	CTCTCACACTCTGCTAGGGA	20	20	45.0	55.0	57.2	57.9	0.0	0.0	0.967983	118441	118677	1316-1316	0.500000	52.3
rps15_p4	rps15	4	TGGCAGTCGTTTCAGTATGT	TCTCTCACACTCTGCTAGGG	20	20	45.0	55.0	57.2	57.9	0.0	0.0	0.967983	118441	118677	1317-1317	0.500000	52.3
rps15_p5	rps15	5	TGGCAGTCGTTTCAGTATGT	TACCGCCACAAATTCCATCC	20	20	45.0	50.0	57.2	58.2	0.0	0.0	1.074003	118441	118677	1265-1265	0.500000	51.4
rps15-ndhI_p1	rps15-ndhI	1	GTCAACGCCTGCTAGCTTAT	TGCTGACTTGACCAGGAAAA	20	20	50.0	45.0	58.1	57.3	17.2	0.0	0.785798	118678	119637	1691-1691	0.500000	53.7
rps15-ndhI_p2	rps15-ndhI	2	GTCAACGCCTGCTAGCTTAT	TTGCTGACTTGACCAGGAAA	20	20	50.0	45.0	58.1	57.3	17.2	0.0	0.785798	118678	119637	1692-1692	0.500000	53.7
rps15-ndhI_p3	rps15-ndhI	3	GTCAACGCCTGCTAGCTTAT	TTTGCTGACTTGACCAGGAA	20	20	50.0	45.0	58.1	57.3	17.2	0.0	0.785798	118678	119637	1693-1693	0.500000	53.7
rps15-ndhI_p4	rps15-ndhI	4	GTCAACGCCTGCTAGCTTAT	TTTTGCTGACTTGACCAGGA	20	20	50.0	45.0	58.1	57.3	17.2	0.0	0.785798	118678	119637	1694-1694	0.500000	53.7
rps15-ndhI_p5	rps15-ndhI	5	GTCAACGCCTGCTAGCTTAT	TCGTGATTCTCACCAACCAA	20	20	50.0	45.0	58.1	57.1	17.2	1.9	0.979399	118678	119637	1471-1471	0.500000	52.2
ndhI_p1	ndhI	1	AGACTTTACCAAAGCTGAGA	TGTAGATCAAAACTGAACCCT	20	21	40.0	38.1	53.9	54.5	0.0	0.0	8.629961	119638	119952	399-399	0.500000	20.0
ndhI_p2	ndhI	2	AGACTTTACCAAAGCTGAGA	TGTAGATCAAAACTGAACCCTAGT	20	24	40.0	37.5	53.9	57.3	0.0	0.0	8.778235	119638	119952	399-399	0.500000	20.0
ndhI_p3	ndhI	3	AGACTTTACCAAAGCTGAGA	AGATCAAAACTGAACCCTAGT	20	21	40.0	38.1	53.9	54.2	0.0	0.0	8.931267	119638	119952	396-396	0.500000	20.0
ndhI_p4	ndhI	4	AGACTTTACCAAAGCTGAGA	ATGTAGATCAAAACTGAACCCT	20	22	40.0	36.4	53.9	55.1	0.0	0.0	9.029921	119638	119952	400-400	0.500000	20.0
ndhI_p5	ndhI	5	AGACTTTACCAAAGCTGAGA	TGTAGATCAAAACTGAACCC	20	20	40.0	40.0	53.9	52.8	0.0	0.0	9.281501	119638	119952	399-399	0.500000	20.0
ndhI-rpl32_p1	ndhI-rpl32	1	TGACGATTCCACAATTCGAA	AGCTTTTAAGGCTGCCCAAT	20	20	40.0	45.0	55.4	58.1	0.0	16.6	2.646107	119953	120964	1154-1154	0.500000	38.8
ndhI-rpl32_p2	ndhI-rpl32	2	TTGACGATTCCACAATTCGA	AGCTTTTAAGGCTGCCCAAT	20	20	40.0	45.0	55.4	58.1	0.0	16.6	2.646107	119953	120964	1155-1155	0.500000	38.8
ndhI-rpl32_p3	ndhI-rpl32	3	GTCGTTTACCAATGTCAGGA	AGCTTTTAAGGCTGCCCAAT	20	20	45.0	45.0	55.4	58.1	0.0	16.6	2.649166	119953	120964	1176-1176	0.500000	38.8
ndhI-rpl32_p4	ndhI-rpl32	4	TTGACGATTCCACAATTCGAA	AGCTTTTAAGGCTGCCCAAT	21	20	38.1	45.0	56.2	58.1	0.0	16.6	2.857879	119953	120964	1155-1155	0.500000	37.1
ndhI-rpl32_p5	ndhI-rpl32	5	GTCGTTTACCAATGTCAGGAA	AGCTTTTAAGGCTGCCCAAT	21	20	42.9	45.0	56.2	58.1	0.0	16.6	2.860314	119953	120964	1176-1176	0.500000	37.1