Hotspot

ycf66

Genome · 4831-5856 · 1026 bp

Back to Sarmentypnum

Pi 0.000975

MarkerSeek score 59.30

Primer available yes

Label ycf66

Feature Metadata

label_name: ycf66
feature_type: gene
region: Genome
length_bp: 1026
variable_sites: 1
pi: 0.000975
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66_p1	TTAAAGCGTGGAGGTTCGAG	AAGTGGTAAGGCAGAGGACT	50.0	50.0	57.9	58.0	2133-2135 bp	1.0	79.2
ycf66_p2	TTAAAGCGTGGAGGTTCGAG	GCGATATGGCCAAGTGGTAA	50.0	50.0	57.9	58.0	2144-2146 bp	1.0	79.1
ycf66_p3	TTAAAGCGTGGAGGTTCGAG	CAAGTGGTAAGGCAGAGGAC	50.0	55.0	57.9	57.9	2134-2136 bp	1.0	78.6
ycf66_p4	GTTCGAGTCCTCTTCAAGGC	AAGTGGTAAGGCAGAGGACT	55.0	50.0	58.3	58.0	2120-2122 bp	1.0	77.5
ycf66_p5	GTTCGAGTCCTCTTCAAGGC	GCGATATGGCCAAGTGGTAA	55.0	50.0	58.3	58.0	2131-2133 bp	1.0	77.5