Hotspot

rps4

Genome · 52453-53061 · 609 bp

Back to Sarmentypnum

Pi 0.00164

MarkerSeek score 59.70

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: Genome
length_bp: 609
variable_sites: 1
pi: 0.00164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	AGGGTACACGACAGAAGGAA	CTACCAATTCCGCCATACCC	50.0	55.0	58.0	58.1	1882 bp	1.0	79.1
rps4_p2	AGGGTACACGACAGAAGGAA	CCGTAGCGTCTACCAATTCC	50.0	55.0	58.0	58.2	1891 bp	1.0	78.8
rps4_p3	AGGGTACACGACAGAAGGAA	GATGGTCATCGGTTCGACTC	50.0	55.0	58.0	58.2	1531 bp	1.0	78.7
rps4_p4	AGGCTTTTGTTTACCGAGGG	CTACCAATTCCGCCATACCC	50.0	55.0	58.1	58.1	1386 bp	1.0	78.4
rps4_p5	AGGGTACACGACAGAAGGAA	TGCTTAGCTCAGAGGTCAGA	50.0	50.0	58.0	57.8	1569 bp	1.0	78.2