Hotspot

rpl16

Genome · 81991-83274 · 1284 bp

Back to Sarmentypnum

Pi 0

MarkerSeek score 60.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: Genome
length_bp: 1284
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGTACAACTACTGCTCGGAC	AACTCCTGGAGATCGCAAAC	55.0	50.0	58.1	57.9	2023-2051 bp	1.0	79.4
rpl16_p2	CGTACAACTACTGCTCGGAC	ACAAAGCCGTATGGAGAACC	55.0	50.0	58.1	57.9	1980-2008 bp	1.0	79.4
rpl16_p3	CGTACAACTACTGCTCGGAC	GCTCGTGTAGAATGGGCTAG	55.0	55.0	58.1	57.9	1812-1840 bp	1.0	79.2
rpl16_p4	ACAGGACCAAAAACACGTGT	AACTCCTGGAGATCGCAAAC	45.0	50.0	58.2	57.9	2137-2165 bp	1.0	78.9
rpl16_p5	ACAGGACCAAAAACACGTGT	ACAAAGCCGTATGGAGAACC	45.0	50.0	58.2	57.9	2094-2122 bp	1.0	78.8