Hotspot

rbcL

Genome · 59581-61008 · 1428 bp

Back to Sarmentypnum

Pi 0.0014
MarkerSeek score 59.60
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
Genome
length_bp
1428
variable_sites
2
pi
0.0014
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 TCAATTGAGTTGTCGGGAGG ACTTTGACAGAAATAAAAGTCAA 50.0 26.1 57.5 52.8 1491 bp 1.0 40.1
rbcL_p2 CAATTGAGTTGTCGGGAGGG ACTTTGACAGAAATAAAAGTCAA 55.0 26.1 58.5 52.8 1490 bp 1.0 40.1
rbcL_p3 AATTGAGTTGTCGGGAGGGA ACTTTGACAGAAATAAAAGTCAA 50.0 26.1 58.6 52.8 1489 bp 1.0 40.1
rbcL_p4 TCAATTGAGTTGTCGGGAGG ACTTACTTTGACAGAAATAAAAGTCA 50.0 26.9 57.5 55.6 1495 bp 1.0 40.1
rbcL_p5 TCAATTGAGTTGTCGGGAGG TGACAGAAATAAAAGTCAAAGAA 50.0 26.1 57.5 52.5 1487 bp 1.0 40.1