Hotspot

ndhH

Genome · 105972-107147 · 1176 bp

Back to Sarmentypnum

Pi 0.00085

MarkerSeek score 51.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: Genome
length_bp: 1176
variable_sites: 1
pi: 0.00085
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	ACTTGTTCTTTAAGATTGGTAGT	ATGAATGAAAAGGAGCGTCA	30.4	40.0	53.6	55.4	1238 bp	1.0	40.1
ndhH_p2	ACTTGTTCTTTAAGATTGGTAGT	TGAATGAAAAGGAGCGTCAT	30.4	40.0	53.6	55.4	1237 bp	1.0	40.1
ndhH_p3	ACTTGTTCTTTAAGATTGGTAGT	AATGAATGAAAAGGAGCGTCA	30.4	38.1	53.6	56.2	1239 bp	1.0	40.1
ndhH_p4	AACTTGTTCTTTAAGATTGGTAGT	ATGAATGAAAAGGAGCGTCA	29.2	40.0	54.4	55.4	1239 bp	1.0	40.1
ndhH_p5	AACTTGTTCTTTAAGATTGGTAGT	TGAATGAAAAGGAGCGTCAT	29.2	40.0	54.4	55.4	1238 bp	1.0	40.1