Hotspot

ndhG

Genome · 102793-103395 · 603 bp

Back to Sarmentypnum

Pi 0.00166
MarkerSeek score 59.70
Primer available yes
Label ndhG

Feature Metadata

label_name
ndhG
feature_type
gene
region
Genome
length_bp
603
variable_sites
1
pi
0.00166
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG_p1 AGTGCTCGAACCATATTACGA TCAGAACAATAGAAACAAAAAGGA 42.9 29.2 56.9 54.9 759 bp 1.0 40.1
ndhG_p2 TGCTCGAACCATATTACGACT TCAGAACAATAGAAACAAAAAGGA 42.9 29.2 56.9 54.9 757 bp 1.0 40.1
ndhG_p3 GTGCTCGAACCATATTACGAC TCAGAACAATAGAAACAAAAAGGA 47.6 29.2 56.9 54.9 758 bp 1.0 40.1
ndhG_p4 AGTGCTCGAACCATATTACGAC TCAGAACAATAGAAACAAAAAGGA 45.5 29.2 58.3 54.9 759 bp 1.0 40.1
ndhG_p5 GTGCTCGAACCATATTACGACT TCAGAACAATAGAAACAAAAAGGA 45.5 29.2 58.3 54.9 758 bp 1.0 40.1