Hotspot

ndhC

Genome · 55690-56061 · 372 bp

Back to Sarmentypnum

Pi 0.00269

MarkerSeek score 44.10

Primer available yes

Label ndhC

Feature Metadata

label_name: ndhC
feature_type: gene
region: Genome
length_bp: 372
variable_sites: 1
pi: 0.00269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC_p1	TGGAGAGTGAATTTATAAGTGGCT	TGTTCTTCTTATTTTACAGGAGT	37.5	30.4	57.7	53.3	470 bp	1.0	40.2
ndhC_p2	TGGAGAGTGAATTTATAAGTGGCT	TTGTTCTTCTTATTTTACAGGAGT	37.5	29.2	57.7	54.1	471 bp	1.0	40.2
ndhC_p3	TGGAGAGTGAATTTATAAGTGGC	TGTTCTTCTTATTTTACAGGAGT	39.1	30.4	56.3	53.3	470 bp	1.0	40.2
ndhC_p4	TGGAGAGTGAATTTATAAGTGGC	TTGTTCTTCTTATTTTACAGGAGT	39.1	29.2	56.3	54.1	471 bp	1.0	40.2
ndhC_p5	GGAGAGTGAATTTATAAGTGGCT	TGTTCTTCTTATTTTACAGGAGT	39.1	30.4	56.1	53.3	469 bp	1.0	40.2