Hotspot

chlL

Genome · 114127-115002 · 876 bp

Back to Sarmentypnum

Pi 0.00228

MarkerSeek score 60.10

Primer available yes

Label chlL

Feature Metadata

label_name: chlL
feature_type: gene
region: Genome
length_bp: 876
variable_sites: 2
pi: 0.00228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL_p1	CCTGTTTCGCATTCAAAAGTGA	TTGATTAATAGGAGGCTTTTTAC	40.9	30.4	58.0	52.5	986 bp	1.0	40.2
chlL_p2	GTGTGGTAATTACCTGTTTCGC	TTGATTAATAGGAGGCTTTTTAC	45.5	30.4	57.9	52.5	998 bp	1.0	40.2
chlL_p3	ACCTGTTTCGCATTCAAAAGT	TTGATTAATAGGAGGCTTTTTAC	38.1	30.4	56.9	52.5	987 bp	1.0	40.2
chlL_p4	CCTGTTTCGCATTCAAAAGTGA	TTTGATTAATAGGAGGCTTTTTAC	40.9	29.2	58.0	53.3	987 bp	1.0	40.2
chlL_p5	CCTGTTTCGCATTCAAAAGTGA	ATTTGATTAATAGGAGGCTTTTT	40.9	26.1	58.0	52.3	988 bp	1.0	40.2