Hotspot

chlB-trnK-UUU

Genome · 27508-28251 · 744 bp

Back to Sarmentypnum

Pi 0.00538
MarkerSeek score 61.80
Primer available yes
Label chlB-trnK-UUU

Feature Metadata

label_name
chlB-trnK-UUU
feature_type
igs
region
Genome
length_bp
744
variable_sites
4
pi
0.00538
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
chlB-trnK-UUU_p1 ACTGAAAAATTTGCGCGTCA AGTCGCACTTAAAAGCCGAG 40.0 50.0 57.5 58.6 873 bp 1.0 71.9
chlB-trnK-UUU_p2 ACTGAAAAATTTGCGCGTCA TAGTCGCACTTAAAAGCCGA 40.0 45.0 57.5 57.3 874 bp 1.0 70.7
chlB-trnK-UUU_p3 ACTGAAAAATTTGCGCGTCA TCGCACTTAAAAGCCGAGTA 40.0 45.0 57.5 57.3 871 bp 1.0 70.7
chlB-trnK-UUU_p4 ACTGAAAAATTTGCGCGTCA CGCACTTAAAAGCCGAGTAC 40.0 50.0 57.5 57.2 870 bp 1.0 70.2
chlB-trnK-UUU_p5 ACTGAAAAATTTGCGCGTCA GTCGCACTTAAAAGCCGAGT 40.0 50.0 57.5 58.9 872 bp 1.0 69.7