Hotspot

trnS-GCU-psbI

Genome · 24464-24569 · 106 bp

Back to Sarmentypnum

Pi 0.0101

MarkerSeek score 62.60

Primer available yes

Label trnS-GCU-psbI

Feature Metadata

label_name: trnS-GCU-psbI
feature_type: igs
region: Genome
length_bp: 106
variable_sites: 1
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbI_p1	GGGTTCGAATCCCTCTCTCT	AATGATCCTGGCCGTAATCC	55.0	50.0	58.2	57.4	157-164 bp	1.0	77.7
trnS-GCU-psbI_p2	GAGATGGCCGAGTGGTTTAA	AATGATCCTGGCCGTAATCC	50.0	50.0	57.6	57.4	219-226 bp	1.0	75.2
trnS-GCU-psbI_p3	AGAGATGGCCGAGTGGTTTA	AATGATCCTGGCCGTAATCC	50.0	50.0	58.4	57.4	220-227 bp	1.0	74.9
trnS-GCU-psbI_p4	GGGTTCGAATCCCTCTCTCT	TCAAATGATCCTGGCCGTAA	55.0	45.0	58.2	56.9	160-167 bp	1.0	73.5
trnS-GCU-psbI_p5	GGGTTCGAATCCCTCTCTCT	AAATGATCCTGGCCGTAATCC	55.0	47.6	58.2	58.1	158-165 bp	1.0	73.1