primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
ycf66_p1	ycf66	1	TTAAAGCGTGGAGGTTCGAG	AAGTGGTAAGGCAGAGGACT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.106042	4831	5856	2133-2135	1.000000	79.2
ycf66_p2	ycf66	2	TTAAAGCGTGGAGGTTCGAG	GCGATATGGCCAAGTGGTAA	20	20	50.0	50.0	57.9	58.0	0.0	11.0	0.112753	4831	5856	2144-2146	1.000000	79.1
ycf66_p3	ycf66	3	TTAAAGCGTGGAGGTTCGAG	CAAGTGGTAAGGCAGAGGAC	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.175152	4831	5856	2134-2136	1.000000	78.6
ycf66_p4	ycf66	4	GTTCGAGTCCTCTTCAAGGC	AAGTGGTAAGGCAGAGGACT	20	20	55.0	50.0	58.3	58.0	0.0	0.0	0.315502	4831	5856	2120-2122	1.000000	77.5
ycf66_p5	ycf66	5	GTTCGAGTCCTCTTCAAGGC	GCGATATGGCCAAGTGGTAA	20	20	55.0	50.0	58.3	58.0	0.0	11.0	0.322213	4831	5856	2131-2133	1.000000	77.5
atpI_p1	atpI	1	GCAAATGATGATGCTCGAGC	TACCTTGACCAATCCCAGGT	20	20	50.0	50.0	58.0	57.9	30.4	0.0	0.087540	18048	18794	1582-1582	1.000000	79.5
atpI_p2	atpI	2	AAATCAAAAGTGGCTTGGCG	TACCTTGACCAATCCCAGGT	20	20	45.0	50.0	57.8	57.9	0.0	0.0	0.207113	18048	18794	1910-1910	1.000000	78.5
atpI_p3	atpI	3	GCAAATGATGATGCTCGAGC	GCAGCAGTACCTTGACCAAT	20	20	50.0	50.0	58.0	58.2	30.4	0.0	0.207408	18048	18794	1589-1589	1.000000	78.5
atpI_p4	atpI	4	TTGCAGGTCGTTGTAATCCC	TACCTTGACCAATCCCAGGT	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.237672	18048	18794	1521-1521	1.000000	78.3
atpI_p5	atpI	5	GCAAATGATGATGCTCGAGC	GCAGTACCTTGACCAATCCC	20	20	50.0	55.0	58.0	58.2	30.4	0.0	0.282356	18048	18794	1586-1586	1.000000	77.9
atpA_p1	atpA	1	AGCGAGAAAGCCGAATGAAT	ACCCTTAGCCTTCCAAGCTA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.062993	21081	22604	2443-2452	1.000000	79.6
atpA_p2	atpA	2	AGCGAGAAAGCCGAATGAAT	CCCACTACCCGCCTTTATTC	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.066666	21081	22604	2406-2415	1.000000	79.6
atpA_p3	atpA	3	AGCGAGAAAGCCGAATGAAT	CGCCGACTATAACCCTTAGC	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.091578	21081	22604	2454-2463	1.000000	79.4
atpA_p4	atpA	4	AGCGAGAAAGCCGAATGAAT	GCGCCGACTATAACCCTTAG	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.091578	21081	22604	2455-2464	1.000000	79.4
atpA_p5	atpA	5	GGGGCAGGCTTAGTTTTTCT	ACCCTTAGCCTTCCAAGCTA	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.108424	21081	22604	2468-2477	1.000000	79.2
trnS-GCU-psbI_p1	trnS-GCU-psbI	1	GGGTTCGAATCCCTCTCTCT	AATGATCCTGGCCGTAATCC	20	20	55.0	50.0	58.2	57.4	17.2	0.0	0.785551	24464	24569	157-164	1.000000	77.7
trnS-GCU-psbI_p2	trnS-GCU-psbI	2	GAGATGGCCGAGTGGTTTAA	AATGATCCTGGCCGTAATCC	20	20	50.0	50.0	57.6	57.4	0.0	0.0	0.965212	24464	24569	219-226	1.000000	75.2
trnS-GCU-psbI_p3	trnS-GCU-psbI	3	AGAGATGGCCGAGTGGTTTA	AATGATCCTGGCCGTAATCC	20	20	50.0	50.0	58.4	57.4	0.0	0.0	1.000119	24464	24569	220-227	1.000000	74.9
trnS-GCU-psbI_p4	trnS-GCU-psbI	4	GGGTTCGAATCCCTCTCTCT	TCAAATGATCCTGGCCGTAA	20	20	55.0	45.0	58.2	56.9	17.2	0.0	1.308027	24464	24569	160-167	1.000000	73.5
trnS-GCU-psbI_p5	trnS-GCU-psbI	5	GGGTTCGAATCCCTCTCTCT	AAATGATCCTGGCCGTAATCC	20	21	55.0	47.6	58.2	58.1	17.2	0.0	1.353120	24464	24569	158-165	1.000000	73.1
chlB_p1	chlB	1	GAGGTTCGAATCCTTCCGTC	TGGGATTCATAAACTTTTCCTACA	20	24	55.0	33.3	58.1	56.0	16.9	0.0	6.062686	25975	27507	2019-2019	1.000000	40.2
chlB_p2	chlB	2	ATCTATTTATGGGGCGTCGC	TGGGATTCATAAACTTTTCCTACA	20	24	50.0	33.3	57.9	56.0	9.2	0.0	6.081347	25975	27507	2076-2076	1.000000	40.2
chlB_p3	chlB	3	GAGGTTCGAATCCTTCCGTC	AATTCTTTTTGTCAATGCTGT	20	21	55.0	28.6	58.1	52.8	16.9	0.0	6.313236	25975	27507	2329-2329	1.000000	40.2
chlB_p4	chlB	4	ATCTATTTATGGGGCGTCGC	AATTCTTTTTGTCAATGCTGT	20	21	50.0	28.6	57.9	52.8	9.2	0.0	6.331897	25975	27507	2386-2386	1.000000	40.2
chlB_p5	chlB	5	GGTAAGGCAGCAGGTTTTGA	TGGGATTCATAAACTTTTCCTACA	20	24	50.0	33.3	58.4	56.0	0.0	0.0	6.370426	25975	27507	2051-2051	1.000000	40.2
chlB-trnK-UUU_p1	chlB-trnK-UUU	1	ACTGAAAAATTTGCGCGTCA	AGTCGCACTTAAAAGCCGAG	20	20	40.0	50.0	57.5	58.6	8.4	6.4	1.054121	27508	28251	873-873	1.000000	71.9
chlB-trnK-UUU_p2	chlB-trnK-UUU	2	ACTGAAAAATTTGCGCGTCA	TAGTCGCACTTAAAAGCCGA	20	20	40.0	45.0	57.5	57.3	8.4	3.0	1.212273	27508	28251	874-874	1.000000	70.7
chlB-trnK-UUU_p3	chlB-trnK-UUU	3	ACTGAAAAATTTGCGCGTCA	TCGCACTTAAAAGCCGAGTA	20	20	40.0	45.0	57.5	57.3	8.4	3.0	1.212273	27508	28251	871-871	1.000000	70.7
chlB-trnK-UUU_p4	chlB-trnK-UUU	4	ACTGAAAAATTTGCGCGTCA	CGCACTTAAAAGCCGAGTAC	20	20	40.0	50.0	57.5	57.2	8.4	0.0	1.265781	27508	28251	870-870	1.000000	70.2
chlB-trnK-UUU_p5	chlB-trnK-UUU	5	ACTGAAAAATTTGCGCGTCA	GTCGCACTTAAAAGCCGAGT	20	20	40.0	50.0	57.5	58.9	8.4	6.4	1.337781	27508	28251	872-872	1.000000	69.7
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	AAAACGGTCTCTTCGTAGCC	20	20	55.0	50.0	58.1	57.9	7.6	0.0	0.140046	40583	41233	793-793	1.000000	79.0
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	AAAACGGTCTCTTCGTAGCC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.162201	40583	41233	796-796	1.000000	78.8
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	GTGGTAGAGTAACGCCATGG	TCTTCGTAGCCAGTCATCCA	20	20	55.0	50.0	58.1	58.2	7.6	0.0	0.216739	40583	41233	784-784	1.000000	78.4
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	TCAGTGGTAGAGTAACGCCA	TCTTCGTAGCCAGTCATCCA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.238894	40583	41233	787-787	1.000000	78.2
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GGCGTAAGTCATCGGTTCAA	AAAACGGTCTCTTCGTAGCC	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.358785	40583	41233	770-770	1.000000	77.2
rps4_p1	rps4	1	AGGGTACACGACAGAAGGAA	CTACCAATTCCGCCATACCC	20	20	50.0	55.0	58.0	58.1	0.0	0.0	0.113161	52453	53061	1882-1882	1.000000	79.1
rps4_p2	rps4	2	AGGGTACACGACAGAAGGAA	CCGTAGCGTCTACCAATTCC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.156274	52453	53061	1891-1891	1.000000	78.8
rps4_p3	rps4	3	AGGGTACACGACAGAAGGAA	GATGGTCATCGGTTCGACTC	20	20	50.0	55.0	58.0	58.2	0.0	0.0	0.163921	52453	53061	1531-1531	1.000000	78.7
rps4_p4	rps4	4	AGGCTTTTGTTTACCGAGGG	CTACCAATTCCGCCATACCC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.202153	52453	53061	1386-1386	1.000000	78.4
rps4_p5	rps4	5	AGGGTACACGACAGAAGGAA	TGCTTAGCTCAGAGGTCAGA	20	20	50.0	50.0	58.0	57.8	0.0	14.0	0.227597	52453	53061	1569-1569	1.000000	78.2
ndhJ_p1	ndhJ	1	AAGCCGGAATAGCTCAGTTG	AGCAAATGCCTGAGCCTAAA	20	20	50.0	45.0	58.0	57.8	5.0	0.9	0.250491	54396	54914	1234-1234	1.000000	78.1
ndhJ_p2	ndhJ	2	AAGCCGGAATAGCTCAGTTG	GGTTGTCCACCAAAACCAGA	20	20	50.0	50.0	58.0	58.2	5.0	0.0	0.259544	54396	54914	1103-1103	1.000000	78.1
ndhJ_p3	ndhJ	3	AAGCCGGAATAGCTCAGTTG	GAGCAAATGCCTGAGCCTAA	20	20	50.0	50.0	58.0	58.2	5.0	0.9	0.274659	54396	54914	1235-1235	1.000000	77.9
ndhJ_p4	ndhJ	4	TGCAGAGACTCAATGGAAGC	AGCAAATGCCTGAGCCTAAA	20	20	50.0	45.0	57.9	57.8	5.0	0.9	0.325659	54396	54914	1485-1485	1.000000	77.5
ndhJ_p5	ndhJ	5	GGGTATGGCGGAATTGGTAG	AGCAAATGCCTGAGCCTAAA	20	20	55.0	45.0	58.1	57.8	0.0	0.9	0.328443	54396	54914	1641-1641	1.000000	77.5
ndhC_p1	ndhC	1	TGGAGAGTGAATTTATAAGTGGCT	TGTTCTTCTTATTTTACAGGAGT	24	23	37.5	30.4	57.7	53.3	0.0	0.0	11.966053	55690	56061	470-470	1.000000	40.2
ndhC_p2	ndhC	2	TGGAGAGTGAATTTATAAGTGGCT	TTGTTCTTCTTATTTTACAGGAGT	24	24	37.5	29.2	57.7	54.1	0.0	0.0	12.168612	55690	56061	471-471	1.000000	40.2
ndhC_p3	ndhC	3	TGGAGAGTGAATTTATAAGTGGC	TGTTCTTCTTATTTTACAGGAGT	23	23	39.1	30.4	56.3	53.3	0.0	0.0	12.319153	55690	56061	470-470	1.000000	40.2
ndhC_p4	ndhC	4	TGGAGAGTGAATTTATAAGTGGC	TTGTTCTTCTTATTTTACAGGAGT	23	24	39.1	29.2	56.3	54.1	0.0	0.0	12.521712	55690	56061	471-471	1.000000	40.2
ndhC_p5	ndhC	5	GGAGAGTGAATTTATAAGTGGCT	TGTTCTTCTTATTTTACAGGAGT	23	23	39.1	30.4	56.1	53.3	0.0	0.0	12.584726	55690	56061	469-469	1.000000	40.2
rbcL_p1	rbcL	1	TCAATTGAGTTGTCGGGAGG	ACTTTGACAGAAATAAAAGTCAA	20	23	50.0	26.1	57.5	52.8	3.4	0.0	8.694394	59581	61008	1491-1491	1.000000	40.1
rbcL_p2	rbcL	2	CAATTGAGTTGTCGGGAGGG	ACTTTGACAGAAATAAAAGTCAA	20	23	55.0	26.1	58.5	52.8	0.0	0.0	8.768544	59581	61008	1490-1490	1.000000	40.1
rbcL_p3	rbcL	3	AATTGAGTTGTCGGGAGGGA	ACTTTGACAGAAATAAAAGTCAA	20	23	50.0	26.1	58.6	52.8	0.0	0.0	8.863547	59581	61008	1489-1489	1.000000	40.1
rbcL_p4	rbcL	4	TCAATTGAGTTGTCGGGAGG	ACTTACTTTGACAGAAATAAAAGTCA	20	26	50.0	26.9	57.5	55.6	3.4	3.1	8.894783	59581	61008	1495-1495	1.000000	40.1
rbcL_p5	rbcL	5	TCAATTGAGTTGTCGGGAGG	TGACAGAAATAAAAGTCAAAGAA	20	23	50.0	26.1	57.5	52.5	3.4	0.0	8.950401	59581	61008	1487-1487	1.000000	40.1
ycf4_p1	ycf4	1	ACCTTCGATTTTCGTTCCTCT	ATTATGGCTTGCCAAGACCG	21	20	42.9	50.0	57.3	58.6	0.0	18.8	2.287234	63085	63639	1258-1258	1.000000	61.9
ycf4_p2	ycf4	2	ACCTTCGATTTTCGTTCCTCT	AGCTCTTTCCAAAGAACGGT	21	20	42.9	45.0	57.3	57.4	0.0	3.4	2.320140	63085	63639	1163-1163	1.000000	61.6
ycf4_p3	ycf4	3	ACCTTCGATTTTCGTTCCTCT	CATTATGGCTTGCCAAGACC	21	20	42.9	50.0	57.3	57.1	0.0	18.8	2.556867	63085	63639	1259-1259	1.000000	59.7
ycf4_p4	ycf4	4	ACCTTCGATTTTCGTTCCTCT	AAGCTCTTTCCAAAGAACGGT	21	21	42.9	42.9	57.3	58.1	0.0	3.4	2.747563	63085	63639	1164-1164	1.000000	58.2
ycf4_p5	ycf4	5	TCCAGCAATTACAATGGCTTC	ATTATGGCTTGCCAAGACCG	21	20	42.9	50.0	56.9	58.6	0.9	18.8	2.756690	63085	63639	1222-1222	1.000000	58.1
petA-psbJ_p1	petA-psbJ	1	TGCATCCGTTATATTAGCACA	GGCTCATACTCTGGATTAGGC	21	21	38.1	52.4	55.2	57.7	0.0	0.0	5.136354	66413	67307	1013-1025	1.000000	40.1
petA-psbJ_p2	petA-psbJ	2	TGCATCCGTTATATTAGCACA	ACTCTGGATTAGGCTCATCGT	21	21	38.1	47.6	55.2	58.3	0.0	2.8	5.137420	66413	67307	1006-1018	1.000000	40.1
petA-psbJ_p3	petA-psbJ	3	TGCATCCGTTATATTAGCACA	ACTCTGGATTAGGCTCATCG	21	20	38.1	50.0	55.2	56.5	0.0	2.8	5.295825	66413	67307	1006-1018	1.000000	40.1
petA-psbJ_p4	petA-psbJ	4	TGCATCCGTTATATTAGCACA	CTCTGGATTAGGCTCATCGT	21	20	38.1	50.0	55.2	56.5	0.0	2.8	5.295825	66413	67307	1005-1017	1.000000	40.1
petA-psbJ_p5	petA-psbJ	5	TGCATCCGTTATATTAGCACAA	GGCTCATACTCTGGATTAGGC	22	21	36.4	52.4	56.0	57.7	0.0	0.0	5.364664	66413	67307	1013-1025	1.000000	40.1
rpl16_p1	rpl16	1	CGTACAACTACTGCTCGGAC	AACTCCTGGAGATCGCAAAC	20	20	55.0	50.0	58.1	57.9	0.0	29.7	0.208137	81991	83274	2023-2051	1.000000	79.4
rpl16_p2	rpl16	2	CGTACAACTACTGCTCGGAC	ACAAAGCCGTATGGAGAACC	20	20	55.0	50.0	58.1	57.9	0.0	4.7	0.216957	81991	83274	1980-2008	1.000000	79.4
rpl16_p3	rpl16	3	CGTACAACTACTGCTCGGAC	GCTCGTGTAGAATGGGCTAG	20	20	55.0	55.0	58.1	57.9	0.0	0.0	0.249067	81991	83274	1812-1840	1.000000	79.2
rpl16_p4	rpl16	4	ACAGGACCAAAAACACGTGT	AACTCCTGGAGATCGCAAAC	20	20	45.0	50.0	58.2	57.9	17.2	29.7	0.271625	81991	83274	2137-2165	1.000000	78.9
rpl16_p5	rpl16	5	ACAGGACCAAAAACACGTGT	ACAAAGCCGTATGGAGAACC	20	20	45.0	50.0	58.2	57.9	17.2	4.7	0.280445	81991	83274	2094-2122	1.000000	78.8
ndhG_p1	ndhG	1	AGTGCTCGAACCATATTACGA	TCAGAACAATAGAAACAAAAAGGA	21	24	42.9	29.2	56.9	54.9	0.0	0.0	9.167094	102793	103395	759-759	1.000000	40.1
ndhG_p2	ndhG	2	TGCTCGAACCATATTACGACT	TCAGAACAATAGAAACAAAAAGGA	21	24	42.9	29.2	56.9	54.9	0.0	0.0	9.167094	102793	103395	757-757	1.000000	40.1
ndhG_p3	ndhG	3	GTGCTCGAACCATATTACGAC	TCAGAACAATAGAAACAAAAAGGA	21	24	47.6	29.2	56.9	54.9	0.0	0.0	9.201255	102793	103395	758-758	1.000000	40.1
ndhG_p4	ndhG	4	AGTGCTCGAACCATATTACGAC	TCAGAACAATAGAAACAAAAAGGA	22	24	45.5	29.2	58.3	54.9	0.0	0.0	9.394151	102793	103395	759-759	1.000000	40.1
ndhG_p5	ndhG	5	GTGCTCGAACCATATTACGACT	TCAGAACAATAGAAACAAAAAGGA	22	24	45.5	29.2	58.3	54.9	0.0	0.0	9.394151	102793	103395	758-758	1.000000	40.1
ndhI_p1	ndhI	1	TCCTTTTTGTTTCTATTGTTCTGA	ACAGCTTCTTTTCAAGTTCT	24	20	29.2	35.0	54.9	52.9	0.0	0.0	12.164871	103495	104046	755-756	1.000000	40.1
ndhI_p2	ndhI	2	TCCTTTTTGTTTCTATTGTTCTGA	AACAGCTTCTTTTCAAGTTCT	24	21	29.2	33.3	54.9	53.9	0.0	0.0	12.245570	103495	104046	756-757	1.000000	40.1
ndhI_p3	ndhI	3	TCCTTTTTGTTTCTATTGTTCTGA	ACAGCTTCTTTTCAAGTTCTT	24	21	29.2	33.3	54.9	53.9	0.0	0.0	12.245570	103495	104046	755-756	1.000000	40.1
ndhI_p4	ndhI	4	TCCTTTTTGTTTCTATTGTTCTGA	AACAGCTTCTTTTCAAGTTCTT	24	22	29.2	31.8	54.9	54.7	0.0	0.0	12.409397	103495	104046	756-757	1.000000	40.1
ndhI_p5	ndhI	5	TCCTTTTTGTTTCTATTGTTCTGA	ACAGCTTCTTTTCAAGTTCTTT	24	22	29.2	31.8	54.9	54.7	0.0	0.0	12.409397	103495	104046	755-756	1.000000	40.1
ndhH_p1	ndhH	1	ACTTGTTCTTTAAGATTGGTAGT	ATGAATGAAAAGGAGCGTCA	23	20	30.4	40.0	53.6	55.4	0.0	0.0	10.039079	105972	107147	1238-1238	1.000000	40.1
ndhH_p2	ndhH	2	ACTTGTTCTTTAAGATTGGTAGT	TGAATGAAAAGGAGCGTCAT	23	20	30.4	40.0	53.6	55.4	0.0	0.0	10.039079	105972	107147	1237-1237	1.000000	40.1
ndhH_p3	ndhH	3	ACTTGTTCTTTAAGATTGGTAGT	AATGAATGAAAAGGAGCGTCA	23	21	30.4	38.1	53.6	56.2	0.0	0.0	10.239472	105972	107147	1239-1239	1.000000	40.1
ndhH_p4	ndhH	4	AACTTGTTCTTTAAGATTGGTAGT	ATGAATGAAAAGGAGCGTCA	24	20	29.2	40.0	54.4	55.4	0.0	0.0	10.252756	105972	107147	1239-1239	1.000000	40.1
ndhH_p5	ndhH	5	AACTTGTTCTTTAAGATTGGTAGT	TGAATGAAAAGGAGCGTCAT	24	20	29.2	40.0	54.4	55.4	0.0	0.0	10.252756	105972	107147	1238-1238	1.000000	40.1
chlL_p1	chlL	1	CCTGTTTCGCATTCAAAAGTGA	TTGATTAATAGGAGGCTTTTTAC	22	23	40.9	30.4	58.0	52.5	0.0	0.0	10.518407	114127	115002	986-986	1.000000	40.2
chlL_p2	chlL	2	GTGTGGTAATTACCTGTTTCGC	TTGATTAATAGGAGGCTTTTTAC	22	23	45.5	30.4	57.9	52.5	30.1	0.0	10.556538	114127	115002	998-998	1.000000	40.2
chlL_p3	chlL	3	ACCTGTTTCGCATTCAAAAGT	TTGATTAATAGGAGGCTTTTTAC	21	23	38.1	30.4	56.9	52.5	0.0	0.0	10.557593	114127	115002	987-987	1.000000	40.2
chlL_p4	chlL	4	CCTGTTTCGCATTCAAAAGTGA	TTTGATTAATAGGAGGCTTTTTAC	22	24	40.9	29.2	58.0	53.3	0.0	0.0	10.692817	114127	115002	987-987	1.000000	40.2
chlL_p5	chlL	5	CCTGTTTCGCATTCAAAAGTGA	ATTTGATTAATAGGAGGCTTTTT	22	23	40.9	26.1	58.0	52.3	0.0	0.0	10.701035	114127	115002	988-988	1.000000	40.2